Glucose (Hk-CP, Axonlab) and free fatty acids (FFAs) (NEFA-HR, WAKO Chemicals) were analyzed enzymatically in EDTA plasma using a Pentra 400 (Horiba). Triglycerides (Sigma), cholesterol (CHOD-PAP, Roche Diagnostics), and HDL cholesterol (CHOD-PAP, Roche Diagnostics), after precipitation of apoB-containing lipoproteins with phosphotungstic acid and magnesium ions, were analyzed in serum also using a Pentra 400. All samples from 1 subject were analyzed within 1 run. LDL cholesterol was calculated for 12 participants according to the Friedewald equation (40 (link)). In a subset of 7 participants (mean ± SD age: 60 ± 3 y; BMI: 30.0 ± 1.7; n = 2 women) inflammatory cytokine concentrations were measured on a Luminex® 200™ system using an inflammation 20-plex human Procartaplex panel (eBioscience, EPX200-12185-901) containing markers for sE-Selectin; intercellular adhesion molecule 1/CD54; IL-1α; IL-4; IL-12p70; IL-17A/cytotoxic T lymphocyte antigen -8; IP-10/CXC chemokine ligand 10; monocyte chemoattractant protein-1/CC chemokine ligand (CCL) 2; macrophage inflammatory protein (MIP) -1α/CCL3; MIP-1β/CCL4; sP-Selectin; and TNF-α.
Pentra 400
Manufactured by Roche
Sourced in Germany
The Pentra 400 is a compact, fully automated clinical chemistry analyzer designed for routine diagnostic testing in medical laboratories. It provides rapid and accurate analysis of a wide range of clinical chemistry parameters from a small sample volume. The Pentra 400 is equipped with advanced technology to ensure reliable and consistent performance.
Automatically generated - may contain errors
2 protocols using pentra 400
1
Comprehensive Metabolic and Inflammatory Panel
2
Plasma and Serum Analyses Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Blood collected in EDTA-coated tubes were immediately stored on ice, centrifuged and plasma was stored at −80 °C until analyses. Blood collected in serum-tubes was stored at room temperature for at least 30 min to allow coagulation, followed by centrifugation and storage at −80 °C until analyses. Glucose (Hk-CP, Axonlab, Amsterdam, The Netherlands) and FFA (NEFA-HR, WAKO chemicals, Neuss, Germany) in meal test samples were analyzed enzymatically in EDTA plasma using a Pentra 400 (Horiba, Montpellier, France). Insulin during the meal test was analyzed using RIA, and insulin during the hyperinsulinemic euglycemic clamp was analyzed using ELISA. Triglycerides (Sigma, Zwijndrecht, The Netherlands), cholesterol (CHOD-PAP, Roche Diagnostics, Mannheim, Germany), and HDL-cholesterol (CHOD-PAP, Roche Diagnostics, Mannheim,Germany) after precipitation of apoB-containing lipoproteins with phosphotungstic acid and magnesium ions, were analyzed in serum also using a Pentra 400. LDL-cholesterol was calculated according the Friedewald equation38 (link).
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