Cox 2 silencer select sirna

SOX2 or YAP1 shRNA pGFP-C-shLenti Vector (SOX2-sh or YAP1-sh) was used for the knockdown of the gene expression (Origene). Non-effective 29-mer scrambled shRNA pGFP-C-shLenti Vector (Origene) was used as a control (SOX2- or YAP1-Ctrl). EF1A-Human-SOX2 lentivirus (SOX2-LV) for SOX2 induction, LentimiRa-GFP-has-let-7 lentivirus (let-7-LV) for let-7 induction, and YAP1 overexpressing lentivirus (YAP1-LV) for YAP1 induction were purchased from Cellomics Technology, Applied Biological Materials, and GenTarget, respectively. EF1A-vector control lentivirus, Lenti-III-mir-GFP control lentivirus, and CMV control lentivirus were used as controls, respectively. For the knockdown of COX2, COX-2 Silencer Select siRNA (Thermo Fisher Scientific) was used.

SOX2 shRNA pGFP-C-shLenti Vector (SOX2-sh) was used for the knockdown of the gene expression (Origene, Rockville, USA). Non-effective 29-mer scrambled shRNA pGFP-C-shLenti Vector (Origene) was used as a control (SOX2-Ctrl). EF1A-Human-SOX2 lentivirus (SOX2-LV) for SOX2 induction was purchased from Cellomics Technology (Rockville, USA). EF1A-vector control lentivirus was used as a control. For the knockdown of COX2, COX-2 Silencer Select siRNA (Thermo Fisher Scientific) was used.