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Dsc 2 stare system

Manufactured by Mettler Toledo
Sourced in Switzerland, Australia

The DSC 2 STARe system is a differential scanning calorimetry (DSC) instrument designed for thermal analysis of materials. It measures the heat flow and temperature differences between a sample and a reference as a function of time or temperature, providing information about the physical and chemical changes that occur in the sample during heating or cooling.

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2 protocols using dsc 2 stare system

1

Differential Scanning Calorimetry Protocol

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DSC analysis was performed in a DSC 2 STARe system simultaneous thermal analyzer with STARe software V13 (Mettler-Toledo, Schwerzenbach, Switzerland) at a heating rate of 10 °C min−1 in the 25 to 300 °C temperature range under a nitrogen flow of 20 mL min−1 and using In as standard.
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2

Thermal Analysis of Milk Fat Formulations

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Thermal analysis of all bulk samples was conducted using a DSC 2 STARe System (Mettler Toledo, Port Melbourne, Australia). Bulk samples were prepared as described above for synchrotron analysis, with ~19-21 mg of sample weighed into a 40 µL aluminium pan (Mettler Toledo, part number ME-27331). All sample pans were hermetically sealed and stored for 48 h at 4 °C. After storage, samples were transferred immediately to a pre-cooled pan holder to maintain temperature and loaded into the DSC chamber set to 4 °C and allowed to equilibrate for 5 min before heating from to 60 °C at 2 °C min -1 . This method is similar to a temperature ramp used by Truong et al. (2015) , except that the heating rate was slowed from 5 °C to 2 °C min -1 to allow for greater resolution during the milk fat melting profile.
The DSC STARe software (version 14.0) was employed to calculate the onset temperature (T onset ), endset (T endset ), along with the maximum values for each peak. The T onset of the last milk fat peak was also calculated, as it shifted due to differences in formulation. All bulk formulations were prepared and analysed in triplicate.
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