Approximately, 5 mg of the powdered grains were used for CHN analysis (CHNS (O) Analyzer, Italy, FLASH EA 1112 series, Thermo finnigan) using the method elaborated by Dumas [21] (link). N and C contents from each genotype was obtained as percentages of the sample weight studied with three biological replications per sample.
Chns o analyzer
Manufactured by Thermo Fisher Scientific
Sourced in United Kingdom
The CHNS/O analyzer is a laboratory instrument designed to determine the elemental composition of organic and inorganic samples. It provides quantitative analysis of carbon, hydrogen, nitrogen, sulfur, and oxygen content in a wide range of sample types.
Automatically generated - may contain errors
Lab products found in correlation
Flash ea 1112 series, by Thermo Fisher Scientific (2 mentions)
Carry eclipse fluorescence spectrophotometer, by Agilent Technologies (1 mentions)
Uv 2250 uv vis spectrophotometer, by Shimadzu (1 mentions)
Fls980 spectrometer, by Edinburgh Instruments (1 mentions)
Icap 6500, by Thermo Fisher Scientific (1 mentions)
400 mhz nmr spectrometer, by Bruker (1 mentions)
Microflex maldi tof mass spectrometer, by Bruker (1 mentions)
D8 advance instrument, by Bruker (1 mentions)
Spectrum 100 series, by PerkinElmer (1 mentions)
6 protocols using chns o analyzer
1
Elemental Analysis of Grains
2
Elemental Composition Analysis of Crushed Grains
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Mature grains were crushed into fine powder and up to 5mg of the same were processed for CHN analysis (CHNS (O) Analyzer, Italy, FLASH EA 1112 series, Thermo finnigan) using the Dumas method (Dumas, 1831) . The content of each element was measured in percentages of the sample weight examined. All measurements were in three replications.
3
Soil and Plant Carbon-Nitrogen Stoichiometry
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Soil and plant samples from each of the seven production systems were analysed for total carbon, oxygen and nitrogen with a CHNS/O analyzer, Thermo Fisher Scientific, Cambridge, UK [31 ]. C:N/C:O stoichiometry parameters for combined CFE system (CFEaverage) is weighted average across the five production systems embedded within the CFE based on 24% acreage each of CFE wheat, CFE barley, CFE oat, CFE ryegrass/Lucerne and 4% of CFE willow.
4
Bone/Dentine Elemental Analysis
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Approximately 1 mg of bone or dentine powder was weighed and packed into a tin capsule for combustion. Carbon (C wt%) and nitrogen (N wt%) contents were measured by a CHNS-O Analyzer (Flash 2000, Thermo Scientific).
5
Comprehensive Analytical Techniques for Chemical Characterization
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1H and 13C NMR spectra were collected on a 400 MHz NMR spectrometer which operated at 400 MHz for 1H and 100 MHz for 13C (Bruker Company). Mass spectra were recorded on a Microflex MALDI-TOF mass spectrometer (Bruker Daltonics). High-resolution mass spectra were obtained from a triple quadrupole GC/MS (Agilent Technologies). Elemental analysis was obtained from a CHNS/O analyzer (Flash 2000, Thermo Scientific). Absorption spectra were obtained from a UV-2250 UV-Vis Spectrophotometer (SHIMADZU, Japan) and emission spectra were obtained from a Carry Eclipse Fluorescence Spectrophotometer (Agilent Technologies). The absolute fluorescence quantum yields and lifetimes were determined using FLS980 Spectrometer (Edinburgh Instruments). The pH buffers were measured from an Ohaus pH meter. Amounts of Hg(ii ) ion in water samples were verified by an inductively coupled plasma-optical emission spectrometer (ICP-OES) (iCAP 6500, Thermo Scientific).
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1H and 13C NMR spectra were collected on a 400 MHz NMR spectrometer which operated at 400 MHz for 1H and 100 MHz for 13C (Bruker Company). Mass spectra were recorded on a Microflex MALDI-TOF mass spectrometer (Bruker Daltonics). High-resolution mass spectra were obtained from a triple quadrupole GC/MS (Agilent Technologies). Elemental analysis was obtained from a CHNS/O analyzer (Flash 2000, Thermo Scientific). Absorption spectra were obtained from a UV-2250 UV-Vis Spectrophotometer (SHIMADZU, Japan) and emission spectra were obtained from a Carry Eclipse Fluorescence Spectrophotometer (Agilent Technologies). The absolute fluorescence quantum yields and lifetimes were determined using FLS980 Spectrometer (Edinburgh Instruments). The pH buffers were measured from an Ohaus pH meter. Amounts of Hg(
6
Comprehensive Materials Characterization Protocol
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X-ray
diffraction (XRD) analysis was carried out using a Bruker D8 Advance
instrument with a Cu Kα source of radiation in 2 theta range
10–90°. Fourier-transform infrared (FTIR) analysis was
carried out using a PerkinElmer Spectrum 100 series with an attenuated
total reflectance accessory in the 4000–400 cm–1 range. Structural morphology was analyzed with the aid of a Zeiss
10 kV field emission scanning electron microscope (FESEM) and high-resolution
transmission electron microscope (HRTEM, JEOL 2100). Energy-dispersive
X-ray (EDX) analysis aided elemental determination and adsorbent mapping.
Pore sizes, pore diameters, and surface area were analyzed with Micromeritics
Tristar II 3020 equipment. Samples were degassed for 12 h at 90 °C.
A Malvern Zetasizer Nanoseries NanoZs with a dip cell was used in
obtaining zeta potential values. Thermogravimetric analysis was carried
out in an inert environment. Elemental analysis was carried out using
a Thermo Scientific CHNS/O analyzer.
diffraction (XRD) analysis was carried out using a Bruker D8 Advance
instrument with a Cu Kα source of radiation in 2 theta range
10–90°. Fourier-transform infrared (FTIR) analysis was
carried out using a PerkinElmer Spectrum 100 series with an attenuated
total reflectance accessory in the 4000–400 cm–1 range. Structural morphology was analyzed with the aid of a Zeiss
10 kV field emission scanning electron microscope (FESEM) and high-resolution
transmission electron microscope (HRTEM, JEOL 2100). Energy-dispersive
X-ray (EDX) analysis aided elemental determination and adsorbent mapping.
Pore sizes, pore diameters, and surface area were analyzed with Micromeritics
Tristar II 3020 equipment. Samples were degassed for 12 h at 90 °C.
A Malvern Zetasizer Nanoseries NanoZs with a dip cell was used in
obtaining zeta potential values. Thermogravimetric analysis was carried
out in an inert environment. Elemental analysis was carried out using
a Thermo Scientific CHNS/O analyzer.
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