Rat anti tubulin sc 53030

Manufactured by Santa Cruz Biotechnology

Rat anti-tubulin (sc-53030) is a primary antibody that detects tubulin, a protein found in the cytoskeleton of cells. This antibody is produced in rats and can be used for various immunoassay applications.

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Lab products found in correlation

Alexa fluor plus 647, by Thermo Fisher Scientific (2 mentions) Ab56416, by Abcam (2 mentions) Mouse anti gfp, by Merck Group (1 mentions) Irdye 800cw goat anti rabbit igg h l, by LI COR (1 mentions) Irdye 800cw, by LI COR (1 mentions) Irdye 680lt, by LI COR (1 mentions)

Spelling variants of this product

Anti-tubulin (154 citations) Sc-53030 (3 citations)

2 protocols using rat anti tubulin sc 53030

Immunoblotting and Immunofluorescence Protocols

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For immunoblotting (IB), all primary antibodies are used at a 1:1,000 dilution, unless stated otherwise. Secondary antibodies are used at a 1:10,000 dilution. For immunofluorescence (IF): primary antibodies were diluted 1:100 and secondary antibodies were used 1:1000. The follow primary antibodies were used: mouse anti-SQSTM1/p62 (ab56416, Abcam), rabbit anti-NDP52 (9036, CST), rabbit anti-ATG9A (13509S, CST), mouse anti-GFP (118114460001, Sigma), rabbit anti-BNIP3 (44060S, CST), rabbit anti-BNIP3L/NIX (12396S, CST), anti-V5 Tag (13202, CST), rat anti-tubulin (sc-53030, Santa Cruz Biotechnology), mouse anti-tubulin (3873S, CST), and mouse anti-EMC3/TMEM111 (67205–1-lg, Proteintech). The following secondary antibodies were used for (IB): goat anti-mouse IgG(H+L) IRDye 680LT (926–68020, LI-COR), goat anti-rabbit IgG(H+L) IRDye800CW (926–32211, LI-COR); secondary antibodies (IF): goat anti-rabbit IgG(H+L) Alexa Fluor Plus 647 (A32733, Invitrogen), goat anti-mouse IgG(H+L) Alexa Fluor Plus 647 (A32728, Invitrogen).

Delgado J.M., Wallace Shepard L., Lamson S.W., Liu S.L, & Shoemaker C.J. (2023). The ER membrane protein complex governs lysosomal turnover of a mitochondrial tail-anchored protein, BNIP3, to restrict mitophagy. bioRxiv.

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Antibody Dilutions for Immunoblotting and Immunofluorescence

Check if the same lab product or an alternative is used in the 5 most similar protocols

For IB, all primary antibodies are used at a 1:1000 dilution, unless stated otherwise. Secondary antibodies are used at a 1:10,000 dilution. For immunofluorescence (IF): primary antibodies were diluted 1:100 and secondary antibodies were used 1:1000. The following primary antibodies were used: mouse anti-SQSTM1/p62 (ab56416, Abcam), rabbit anti-NDP52 (9036, CST), rabbit anti-ATG9A (13509S, CST), mouse anti-GFP (118114460001, Sigma), rabbit anti-BNIP3 (44060S, CST), rabbit anti-BNIP3L/NIX (12396S, CST), anti-V5 Tag (13202, CST), rat anti-tubulin (sc-53030, Santa Cruz Biotechnology), mouse anti-tubulin (3873S, CST), and mouse anti-EMC3/TMEM111 (67205-1-lg, Proteintech). The following secondary antibodies were used for (IB): goat anti-mouse IgG(H+L) IRDye 680LT (926-68020, LI-COR), goat anti-rabbit IgG(H+L) IRDye800CW (926-32211, LI-COR); secondary antibodies (IF): goat anti-rabbit IgG(H+L) Alexa Fluor Plus 647 (A32733, Invitrogen), goat anti-mouse IgG(H+L) Alexa Fluor Plus 647 (A32728, Invitrogen).

Delgado J.M., Shepard L.W., Lamson S.W., Liu S.L, & Shoemaker C.J. (2023). The ER membrane protein complex restricts mitophagy by controlling BNIP3 turnover. The EMBO Journal, 43(1), 32-60.

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