Horseradish peroxidase hrp conjugated goat anti rabbit secondary antibody

Anti-HOXB8 (1: 1000 dilution; cat. no. ab125727), anti-c-Myc (1: 1000 dilution; cat. no. ab32072), anti-CyclinD1 (1: 1000 dilution; cat. no. ab134175), anti-E-cadherin (1: 1000 dilution; cat. no. ab15148), anti-MMP2 (1: 1000 dilution; cat. no. ab92536), anti-vimentin (1: 1000 dilution; cat. no. ab92547), anti-Bax (1: 1000 dilution; cat. no. ab32503), anti-bcl-2 (1: 1000 dilution; cat. no. ab182858) and anti-tubulin (1: 1000 dilution, cat. no. ab6046) antibodies were obtained from Abcam (San Francisco, CA, USA). Control shRNA, and HOXB8 shRNA clones were obtained from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody (1: 1000; cat no. A0208) and DAB (0.05–0.03%) horseradish peroxidase color development kits (cat no. ST033) were obtained from Beyotime Institute of Biotechnology (Haimen, China). Lipofectamine™ 2000, Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Invitrogen (Thermo Fisher Scientific, Inc., Waltham, MA, USA). SuperSignal™ West Pico PLUS Chemiluminescent substrate was purchased from Thermo Fisher Scientific, Inc. Penicillin G and streptomycin were purchased from Sangon Biotechnology Co., Ltd. (Shanghai, China).

Restriction endonuclease enzymes (EcoRI, BamHI and SalI), DNA polymerase Pfu, DNA marker, gel extraction kits, T4 DNA ligase, the PureLink RNA kit, and the cDNA SuperScript First Strand Synthesis kits were purchased from TaKaRa (Shiga, Japan). pGEX-4T-1, Escherichia coli DH5α, and E. coli Rosseta cells were maintained in our laboratory. Glutathione agarose was purchased from Thermo Fisher Scientific Inc. (Waltham, Massachusetts, USA). Anti-IFN-α2a antibody was purchased from Gene Tex Inc. (Irvine, California, USA). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit secondary antibody and DAB color development kits were obtained from Beyotime Biotechnology Company (Hangzhou, China). Reverse transcriptase PCR kits, RNA extraction kits, Lipofectamine 2000, Dulbecco’s modified Eagle’s medium (DMEM), and fetal bovine serum were purchased from Invitrogen (Life Technologies, Grand Island, New York, USA). West Pico ECL reagent was purchased from Pierce Biotechnology Inc. (Rockford, Illinois, USA). Penicillin G and streptomycin were purchased from Shanghai Biotechnology Company (Shanghai, China). PCR primers were synthesized by Shanghai Sangon Biotechnology Company (Shanghai, China).