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Recombinant human c5a

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Recombinant human C5a is a laboratory reagent used in research applications. It is a protein fragment derived from the complement component C5. C5a is a potent anaphylatoxin and chemoattractant, playing a role in the immune response.

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Lab products found in correlation

Lysophosphatidic acid, by Santa Cruz Biotechnology (1 mentions) Phospho pras40, by Cell Signaling Technology (1 mentions) Pras40, by Cell Signaling Technology (1 mentions) Phospho gsk3β, by Cell Signaling Technology (1 mentions) Phospho foxo1, by Cell Signaling Technology (1 mentions) Prolong gold mounting medium with dapi, by Thermo Fisher Scientific (1 mentions) Recombinant human pdgf bb, by R&D Systems (1 mentions) Eea1 antibody, by BD (1 mentions) Anti mouse alexa 488, by Thermo Fisher Scientific (1 mentions) Lamp 1 antibody, by Abcam (1 mentions) 4e bp1, by Cell Signaling Technology (1 mentions) Foxo3a, by Cell Signaling Technology (1 mentions) Gsk3β, by Cell Signaling Technology (1 mentions) Foxo1, by Cell Signaling Technology (1 mentions) Phospho akt, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) P70s6k, by Cell Signaling Technology (1 mentions) Phospho p70s6k, by Cell Signaling Technology (1 mentions) Hl 60 cells, by American Type Culture Collection (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions)

3 protocols using recombinant human c5a

1

Comprehensive Antibody Toolkit for Cellular Signaling

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The following antibodies were purchased from Cell Signaling Technology: Akt(#4691), Phospho-Akt(Thr308)(#4056), Phospho-Akt(Ser473)(#4060), GAPDH, GSK-3β(#9315), Phospho-GSK-3β(#9323), FoxO1(#2880), Phospho-FoxO1 (Thr24)/FoxO3a(Thr32)/FoxO4(Thr28)(#2599), PRAS40(#2691), Phospho-PRAS40 (Thr246)(#2997), 4E-BP1(#9644), p70 S6K(#2708), Phospho-p70 S6K (Thr389)(#9234). SQSTM1/p62 (D5E2)(#8025), LC3B(#12513). PARP(#9532), Caspase-3(#9665), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204)(#4377), p44/42 MAPK (Erk1/2) (#4695), Phospho-NF-ΚB P65 (Ser 536)(#3033), NF-ΚB P65(#4764). Lysophosphatidic Acid (#sc-201053, Santa Cruz), Recombinant Human PDGF-BB (#220-BB, R&D), Recombinant Human C5a (Peprotech, #300-70), goat anti-IgM (#5C07615, Meridian). LC3B antibody (#3868, CST). Tubulin antibody (#8035, Santa Cruz). EEA1 antibody (#610456, BD Bioscience) and LAMP1 antibody (#24170, Abcam). Secondary antibodies, Alexa488-anti-mouse, Alexa594-anti-rabbit and Prolong gold mounting medium with DAPI were purchased from Invitrogen.
Liu X., Wang A., Liang X., Liu J., Zou F., Chen C., Zhao Z., Deng Y., Wu H., Qi Z., Wang B., Wang L., Liu F., Xu Y., Wang W., Fernandes S.M., Stone R.M., Galinsky I.A., Brown J.R., Loh T., Griffin J.D., Zhang S., Weisberg E.L., Zhang X., Liu J, & Liu Q. (2016). Simultaneous inhibition of Vps34 kinase would enhance PI3Kδ inhibitor cytotoxicity in the B-cell malignancies. Oncotarget, 7(33), 53515-53525.
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2

Differentiation and C5a-mediated MCP-1 Analysis

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To differentiate to neutrophil-like, HL-60 cells (ATCC) were cultured in RPMI1640 containing 10% fetal bovine serum and 1.3% DMSO for 5days (Millius and Weiner, 2009). Differentiated cells were treated with 3nM of recombinant human C5a (PeproTech, inc). After 1hour, these cells were harvested and isolated mRNA to analyze MCP-1 expression by quantitative RT-PCR.
Osaka M., Ito S., Honda M., Inomata Y., Egashira K, & Yoshida M. (2016). Critical role of the C5a-activated neutrophils in high-fat diet-induced vascular inflammation. Scientific Reports, 6, 21391.
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3

Differentiation and C5a Stimulation of HL-60 Leukemia Cells

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Human monocytic leukemia cell line (THP‐1) was obtained from RIKEN BioResource Research Center Cell Bank (Tsukuba, Japan) and was maintained in RPMI‐1640 (FUJIFILM Wako Pure Chemical Corporation, Osaka, Japan) culture medium containing 10% fetal bovine serum (FBS), 100 IU/ml penicillin, and 0.17 mmol/L streptomycin (Gibco, Life Technologies Japan, Tokyo, Japan). The human leukemia cell line HL‐60 (ATCC) was cultured in RPMI‐1640 containing 10% FBS and penicillin/streptomycin. To differentiate HL‐60 cells, 1.3% dimethyl sulfoxide (DMSO; FUJIFILM Wako Pure Chemical Corporation) was added to the culture medium, and they were cultured for 5 days in an incubator maintained at 37°C with 5% CO2 [3, 9].
Differentiated HL‐60 (dHL‐60, 1 × 106 cells) cells were stimulated by 3 nm recombinant human C5a (PeproTech Inc. NJ, USA) followed by each experiment.
Dewan S.M., Osaka M., Deushi M, & Yoshida M. (2021). Complement C5a‐triggered differentiated HL‐60 stimulates migration of THP‐1 monocytic leukocytes via secretion of CCL2. FEBS Open Bio, 11(5), 1374-1381.
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