After local anesthesia with lidocaine/bupivacaine, skeletal muscle samples were collected using the Bergstrom technique with suction from the vastus lateralis (Propper Manufacturing Co., Long Island City, NY). Two separate incisions were made to collect tissues at baseline and post-exercise. The second biopsy collections were obtained within a time frame of no more than 3 minutes following the completion of exercise. All skeletal muscle samples were visually assessed and cleared for intramuscular adipose tissue, and then immediately snap frozen in liquid nitrogen for mRNA and Protein measures. Samples were blotted dry and then mounted in a mixture of Optimal Cutting Temperature (OCT, Thermo Scientific, Waltham, MA) and Tragacanth powder (Acros, Geel, Belgium) for immunohistochemical measures of glycogen, intramyocellular lipid, and fiber typing. Another sample was collected for measurements of ex vivo palmitate oxidation.
Optimal cutting temperature oct
Manufactured by Thermo Fisher Scientific
Sourced in United States
Optimal Cutting Temperature (OCT) is a medium used for embedding and freezing tissue samples for cryosectioning. It provides a stable and uniform support for the tissue during the sectioning process, enabling thin and consistent slices to be prepared for further analysis or examination.
Automatically generated - may contain errors
Lab products found in correlation
Ripa lysis buffer, by Thermo Fisher Scientific (2 mentions)
Tragacanth powder, by Thermo Fisher Scientific (1 mentions)
Protease inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)
Cox 2, by ABclonal (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Gapdh, by ABclonal (1 mentions)
P ampk, by ABclonal (1 mentions)
2 protocols using optimal cutting temperature oct
1
Skeletal Muscle Biopsy and Analysis
2
6-OHDA-induced Neuroinflammation Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
VPA was obtained from Sigma-Aldrich (Saint Louis, MO, United States of America (USA)). 6-OHDA, L-ascorbic acid and LPS were obtained from (Medchemeexpress, USA). Ketamine and xylazine was obtained from Prince Naif bin Abdul-Aziz Health Research Center, King Saud University (KSU), Saudi Arabia (SA). Optimal Cutting Temperature (OCT) was obtained from (ThermoFisher Scientific, USA). Trizol reagent was obtained from (Invitrogen, USA). SYBR Green, PCR Master Mix, and cDNA reverse transcription kit were bought from (Medchemeexpress, USA). IL-6, TNF-α, and NF-κB primers were created and customized by (integrated DNA Technologies (IDT), Belgium). Cocktail protease inhibitors and RIPA lysis buffer were purchased from (ThermoFisher Scientific, USA). Bicinchoninic acid assay (BCA assay) was purchased from (Molequle-on, Auckland, New Zealand). Primary rabbit polyclonal antibodies COX-2, GPx-1, p-AMPK, AMPK, and GAPDH and secondary horseradish peroxidase goat antirabbit IgG antibodies were purchased from (ABclonal Technology, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!