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Urokinase type plasminogen activator u pa

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Urokinase-type plasminogen activator (u-PA) is a laboratory reagent used in research applications. It is a serine protease that catalyzes the conversion of plasminogen to plasmin, a key enzyme involved in fibrinolysis and extracellular matrix remodeling.

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Lab products found in correlation

Human fibrinogen, by Merck Group (1 mentions) Thrombin, by Merck Group (1 mentions) Fxiiia, by Merck Group (1 mentions) Trizma hcl, by Merck Group (1 mentions) Alexa fluor 488 conjugated fibrinogen, by Thermo Fisher Scientific (1 mentions) Trizma base, by Merck Group (1 mentions)

Spelling variants of this product

Plasminogen (35 citations) Urokinase plasminogen activator (uPA) (5 citations) Urokinase (5 citations) Urokinase-type plasminogen activator (2 citations)

2 protocols using urokinase type plasminogen activator u pa

1

Fibrinolysis Assay Protocol

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Human fibrinogen, thrombin, FXa, FXIIIa, urokinase-type plasminogen activator (u-PA), trizma base, and trizma HCl were purchased from Sigma-Aldrich (St. Louis, MO, USA). Paranitroaniline chromogenic substrates were obtained from Chromogenix (Milan, Italy). Alexa Fluor 488-conjugated fibrinogen was purchased from Invitrogen (Eugene, OR, USA). Other reagents used were of analytical grade and purchased from commercial sources.
Choi J.H, & Kim S. (2022). In Vitro Antithrombotic, Hematological Toxicity, and Inhibitor Studies of Protocatechuic, Isovanillic, and p-Hydroxybenzoic Acids from Maclura tricuspidata (Carr.) Bur. Molecules, 27(11), 3496.
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2

Plasmin Activity Assay with GAPDH

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Briefly, 20 μg of recombinant GAPDH proteins was incubated with 150 nM human plasminogen, 0.5 U urokinase-type plasminogen activator (uPA; Sigma-Aldrich), and 0.2 mM chromogenic substrate of plasmin (MyBioSource, San Diego, CA, USA). Increase in plasmin activity was assessed at 30-min intervals by measuring absorbance at 405 nm for 3 h. In inhibition tests, 10 mM EACA was added.
Chen P.C., Hsieh M.H., Kuo W.S., Wu L.S., Kao H.F., Liu L.F., Liu Z.G., Jeng W.Y, & Wang J.Y. (2022). Moonlighting glyceraldehyde-3-phosphate dehydrogenase (GAPDH) protein of Lactobacillus gasseri attenuates allergic asthma via immunometabolic change in macrophages. Journal of Biomedical Science, 29, 75.
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