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Anti erm 3142

Manufactured by Cell Signaling Technology
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Anti-ERM (#3142) is a primary antibody product offered by Cell Signaling Technology. It is designed for the detection of ERM proteins, which include ezrin, radixin, and moesin, in various applications.

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Lab products found in correlation

Bca protein assay kit, by Thermo Fisher Scientific (4 mentions) Bis tris gel, by Thermo Fisher Scientific (2 mentions) Protease and phosphatase inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Supersignal west dura chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Anti rabbit igg conjugated to hrp, by Cell Signaling Technology (2 mentions) β actin clone ac 15, by Merck Group (1 mentions) Pp1α c 19, by Santa Cruz Biotechnology (1 mentions) Gfp trap, by Proteintech (1 mentions) Anti mcherry, by Abcam (1 mentions) Tetramethylrhodamine isothiocyanate tritc phalloidin, by Merck Group (1 mentions) Anti ha clone 6e2, by Cell Signaling Technology (1 mentions) Anti gfp jl 8, by Takara Bio (1 mentions) Anti γ tubulin gtu 88, by Merck Group (1 mentions) α tubulin clone dm1a, by Merck Group (1 mentions) Flag m2, by Merck Group (1 mentions)

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Anti-ERM (5 citations)

3 protocols using anti erm 3142

1

Antibody-based Western Blotting and Immunofluorescence

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The following antibodies were used for Western blotting (WB) and IF: anti–γ-tubulin GTU88 (IF), α-tubulin clone DM1A (IF+WB), α-tubulin clone YL 1/2 (IF), β-actin clone AC15 (IF), GFP (WB), PPP1R13L HPA041231 (WB), myosin 1c HPA 001768 (IF, WB), FLAG M2 (WB) from Sigma-Aldrich; anti-mCherry (WB) and NuMA [EP3976] (WB) from Abcam; anti-iASPP 18590–1-AP (WB) from Proteintech and PCRP-PPP1R13L-2G4 (WB) from Developmental Studies Hybridoma Bank; anti–ERM #3142 (WB), anti-EB1 clone 5 (WB), and anti-HA clone 6E2 (WB) from Cell Signaling Technology; anti-EB1 KT51 (IF), myosin Ic (13; WB), PP1α (C-19; WB) from Santa Cruz Biotechnology; anti-CDK5RAP2, A300-554 (IF) from Bethyl; anti–Phospho-Histone H3 (Ser10) from Millipore (WB); and anti-GFP JL-8 from Clontech (for GFP1-10, WB). GFP-Trap was from Chromotek, anti-HA agarose conjugated beads, tetramethylrhodamine isothiocyanate (TRITC)-phalloidin from Sigma-Aldrich, and Strep-Tactin beads from IBA.
Mangon A., Salaün D., Bouali M.L., Kuzmić M., Quitard S., Thuault S., Isnardon D., Audebert S., Puech P.H., Verdier-Pinard P, & Badache A. (2021). iASPP contributes to cell cortex rigidity, mitotic cell rounding, and spindle positioning. The Journal of Cell Biology, 220(12), e202012002.
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2

FRC Protein Extraction and Western Blot

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Purified FRCs were plated in 10 cm dishes at a density of 3 × 105 cells per plate overnight. Cells were serum-starved for 5 h and then were lysed in lysis buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol; Thermo Scientific) containing protease and phosphatase inhibitor cocktails (Thermo Scientific). Protein concentration was determined with the BCA protein assay kit (Thermo Scientific). Equal amounts of protein were loaded onto 4–12% Bis-Tris gels (Life Technologies) and then transferred to a nitrocellulose membrane. After blocking in 5% non-fat milk for 1 h, the membranes were incubated with primary antibodies in 5% BSA overnight. The following primary antibodies were used: anti-ERM (#3142), anti-p-ERM (#3149), anti-MLC (#8505), and anti-p-MLC (S19, #3671) (all from Cell Signaling Technology). After 3 washes with TBS, the membranes were incubated with anti-rabbit-IgG conjugated to HRP (Cell Signaling Technology) for two h at 25 °C. Finally, the signal was visualized with the SuperSignal WestDura Chemiluminescent Substrate (Fisher).
Astarita J.L., Cremasco V., Fu J., Darnell M.C., Peck J.R., Nieves-Bonilla J.M., Song K., Woodruff M.C., Gogineni A., Onder L., Ludewig B., Weimer R.M., Carroll M.C., Mooney D.J., Xia L, & Turley S.J. (2014). The CLEC-2–podoplanin axis controls fibroblastic reticular cell contractility and lymph node microarchitecture. Nature immunology, 16(1), 75-84.
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3

FRC Protein Extraction and Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
Purified FRCs were plated in 10 cm dishes at a density of 3 × 105 cells per plate overnight. Cells were serum-starved for 5 h and then were lysed in lysis buffer (25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol; Thermo Scientific) containing protease and phosphatase inhibitor cocktails (Thermo Scientific). Protein concentration was determined with the BCA protein assay kit (Thermo Scientific). Equal amounts of protein were loaded onto 4–12% Bis-Tris gels (Life Technologies) and then transferred to a nitrocellulose membrane. After blocking in 5% non-fat milk for 1 h, the membranes were incubated with primary antibodies in 5% BSA overnight. The following primary antibodies were used: anti-ERM (#3142), anti-p-ERM (#3149), anti-MLC (#8505), and anti-p-MLC (S19, #3671) (all from Cell Signaling Technology). After 3 washes with TBS, the membranes were incubated with anti-rabbit-IgG conjugated to HRP (Cell Signaling Technology) for two h at 25 °C. Finally, the signal was visualized with the SuperSignal WestDura Chemiluminescent Substrate (Fisher).
Astarita J.L., Cremasco V., Fu J., Darnell M.C., Peck J.R., Nieves-Bonilla J.M., Song K., Woodruff M.C., Gogineni A., Onder L., Ludewig B., Weimer R.M., Carroll M.C., Mooney D.J., Xia L, & Turley S.J. (2014). The CLEC-2–podoplanin axis controls fibroblastic reticular cell contractility and lymph node microarchitecture. Nature immunology, 16(1), 75-84.
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