The generation of Stk4 KO mice and the flow cytometry experiments follow the protocols that we have described elsewhere [11 (link)]. For the Complete Blood Count (CBC) analysis, blood was collected into EDTA-coated MiniCollect blood collection tubes (Greiner Bio-One, Kremsmünster, Austria, catalog #VG-450474) via submandibular vein puncture from unanesthetized mice. 40 μL of blood from each mouse were aliquoted and analyzed by a Hemavet 950FS (Drew Scientific, Oxford, CT, USA). Using an electrical impedance and focused flow cell system, the Hemavet quantified the total number of white blood cells, eosinophils, neutrophils, basophils, and lymphocytes.
Minicollect blood collection tubes
Manufactured by Greiner
Sourced in Austria
MiniCollect blood collection tubes are designed for the collection and transportation of capillary blood samples. The tubes feature a small diameter and are available in various volumes to accommodate different sample needs.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using minicollect blood collection tubes
1
Complete Blood Count Analysis in Mice
2
Blood collection from Sunda pangolin
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Blood was collected from the coccygeal vein along the ventral midline of the tail (Fig. 1 ![]()
ml of whole blood was collected using a 10 ml syringe
(Terumo Co., Tokyo, Japan) with a 21 G × 1.5” needle (Terumo Co.). Blood for hematology
(500 µl) and serum biochemistry studies (3 ml) was
collected in MiniCollect® blood collection tubes (Greiner Bio-One,
Kremsmünster, Upper Austria, Austria) containing ethylene diaminetetraacetic acid (EDTA)
and plain blood collection tubes, respectively. Immediately after collection a blood smear
was produced, air-dried, and stored for analysis. Sample quality was recorded at the time
of sampling and before analyses. Samples with signs of lipemia or hemolysis were excluded
from the study. All samples were processed within 3 hr of collection, apart from blood
smears which were stained and analyzed the following day.
Blood collection from the coccygeal vein on the ventral aspect of the tail of a
Sunda pangolin under general anesthesia.
ml of whole blood was collected using a 10 ml syringe
(Terumo Co., Tokyo, Japan) with a 21 G × 1.5” needle (Terumo Co.). Blood for hematology
(500 µl) and serum biochemistry studies (3 ml) was
collected in MiniCollect® blood collection tubes (Greiner Bio-One,
Kremsmünster, Upper Austria, Austria) containing ethylene diaminetetraacetic acid (EDTA)
and plain blood collection tubes, respectively. Immediately after collection a blood smear
was produced, air-dried, and stored for analysis. Sample quality was recorded at the time
of sampling and before analyses. Samples with signs of lipemia or hemolysis were excluded
from the study. All samples were processed within 3 hr of collection, apart from blood
smears which were stained and analyzed the following day.
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