4-Cyanobenzaldehyde, benzil, 4-bromobenzoic acid, bis(pinacolato)diboron, 1,1′-carbonyldiimidazole (CDI), [1,1′-bis(diphenylphosphino)ferrocene]dichloropalladium(II)dichloride (PdCl2 (dppf)), H2O2, potassium super-oxide (KO2), t-butyl hydroperoxide (TBHP), m-chloroperoxybenzoic acid (mCPBA), hypochlorous acid (HOCl), HEPES buffer solution, Whatman papers, Dulbecco's phosphate-buffered saline (DBS), Roswell Park Memorial Institute (RPMI) medium, fetal bovine serum (FBS), penicillin, streptomycin, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ammonium acetate, acetic acid, ethanol, hydroxylamine hydrochloride, sodium hydroxide, ethyl acetate, hexane, sodium bicarbonate, dimethylformamide (DMF), dimethyl sulfoxide (DMSO), 1,4-dioxane, and potassium acetate were purchased from Daejung Chemicals (Siheungsi, Kyunggido, Republic of Korea). Sodium nitrate (NaNO3−), potassium perchlorate (KClO4−), sodium sulfate (Na2SO4), sodium nitrite (NaNO2), and benzoyl peroxide (BPO) were obtained from AccuStandard (New Haven, CT, USA). The MCF-7 cells were purchased from the Korean Cell Line Bank (Seoul, Republic of Korea). All reagents were used as received without further purification.
Benzil
Manufactured by Merck Group
Sourced in United States, Germany
Benzil is a chemical compound used as a laboratory reagent. It is a white, crystalline solid with a melting point of 95°C. Benzil is commonly used in organic synthesis reactions as a source of benzoyl groups.
Automatically generated - may contain errors
Lab products found in correlation
Sodium hydroxide (naoh), by Daejung Chemicals (1 mentions)
4 cyanobenzaldehyde, by Merck Group (1 mentions)
Mcf 7 cells, by Korean Cell Line Bank (1 mentions)
M chloroperoxybenzoic acid, by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Acetic acid, by Daejung Chemicals (1 mentions)
Ethanol, by Daejung Chemicals (1 mentions)
Potassium superoxide, by Merck Group (1 mentions)
Hydroxylamine hydrochloride, by Daejung Chemicals (1 mentions)
Hypochlorous acid, by Merck Group (1 mentions)
Hepes buffer solution, by Merck Group (1 mentions)
T butyl hydroperoxide, by Merck Group (1 mentions)
Sodium bicarbonate, by Daejung Chemicals (1 mentions)
Ammonium acetate, by Daejung Chemicals (1 mentions)
Ethyl acetate, by Daejung Chemicals (1 mentions)
Whatman, by Cytiva (1 mentions)
Nicotinic hydrazide, by Merck Group (1 mentions)
6 protocols using benzil
1
Cytotoxicity Assay of Chemical Compounds
2
Synthesis and Characterization of Ligand
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Materials used for the synthesis of the ligand was of analytical grade and used without any purification. Benzil, Nicotinic hydrazide were purchased from Sigma Aldrich and used without any purification. FTIR of the synthesized ligand was obtained using PerkinElmer IR Version 10.6.0 in the region of 4000 cm−1 – 400 cm−1. UV of the ligand was recorded using UV-2600 series spectrometer in the range of 200–600 nm using ethanol as solvent.1H NMR and 13C NMR was recorded using Bruker Advance 400 MHz FT-NMR Spectrometer using DMSO as solvent. Molecular docking was performed using CB dock tool. DFT calculations were done using Gaussian 16 W software and visualised using Gauss View 6.1 software. Invitro anticancer studies were done using MCF cell lines collected from National Centre for Cell Sciences (NCCS), Pune, India.
3
Synthesis and Characterization of DTTCT and Copper Complex
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The syntheses of dibenzo[e,k]-2,3,8,9-tetraphenyl-1,4,7,10-tetraaza-cyclododeca-1,3,7,9tetraene (DTTCT), its copper complex, and the coupon samples were performed following the method described elsewhere [9] . In brief, benzil (0.05 mol; Aldrich 98%) and o-phenylenediamine (0.05 mol; Aldrich, 99.5%) were refluxed in ethanol with few drops of hydrochloric acid (Ajax Finechem, 32%) for 6 hours at 80 °C. The resultant DTTCT (0.01 mol) was mixed with copper acetate monohydrate (0.05 mol; Ajax APS) in ethanol and refluxed at 80 °C for 6 hours.
The coupons were synthesized by mixing the copper complex (2 mg) with pre-dissolved PVC (Chemson Pacific Pty Ltd) in tetrahydrofuran (THF, Chem-Supply) solution (0.3 mL, 66 mg/L) in an ultrasonic bath. Round glass cover slips (18 mm diameter, ProSciTech) were washed with dilute nitric acid, acetone, and ethanol followed by overnight drying at 110 °C before being used as the cast template. Bare coupon (PVC without any copper complex, typical weight ~20 mg) was used as the control. The coupons were dried at 50 °C for 12 hours and peeled off from the glass cover slips. Characterizations of the ligand, complex, and coupons have been presented elsewhere [9] .
The coupons were synthesized by mixing the copper complex (2 mg) with pre-dissolved PVC (Chemson Pacific Pty Ltd) in tetrahydrofuran (THF, Chem-Supply) solution (0.3 mL, 66 mg/L) in an ultrasonic bath. Round glass cover slips (18 mm diameter, ProSciTech) were washed with dilute nitric acid, acetone, and ethanol followed by overnight drying at 110 °C before being used as the cast template. Bare coupon (PVC without any copper complex, typical weight ~20 mg) was used as the control. The coupons were dried at 50 °C for 12 hours and peeled off from the glass cover slips. Characterizations of the ligand, complex, and coupons have been presented elsewhere [9] .
4
Synthesis of Benzimidazole Derivatives
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Benzil (98%) was purchased from Aldrich Chemical. o-Phenylenediamine (98%) was purchased from Alfa Aesar. Both were used without further purification.
5
Synthesis and characterization of FTC prodrugs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anhydrous 1,6-hexamethylene bis(chloroformate) (95%) and trimethylolpropane tri(chloroformate) (97%) were purchased from Manchester Organics Ltd (UK) and used as received. 4-Dimethylaminopyridine (DMAP), anhydrous pyridine, anhydrous dimethylsulfoxide (DMSO) and anhydrous dichloromethane (DCM) were purchased from Merck (UK) and used as received. N,N′-Dimethylformamide (DMF), ethanol, ethyl acetate, hexane, CDCl3, and magnesium sulfate (MgSO4) were purchased from Fischer Scientific, whilst anhydrous ethanol and hexyl chloroformate was purchased from Sigma Aldrich. Emtricitabine (FTC) was purchased from WISCHEM (China) and used without further purification. Male and female human plasma and the dipotassium salt of ethylenediaminetetraacetic acid (K2-EDTA) were purchased from BioIVT and the plasma samples were pooled. Benzil was purchased from Merck (Germany). Pooled, mixed gender, adult human liver microsome and phosphate buffered saline (PBS) at pH 7.4 was purchased from ThermoFisher Scientific (USA). Carboxylesterase 1 (CES1) specific activity assay kit was purchased from Abcam (UK).
6
Pharmacological Modulation of Cellular Processes
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!