Anti phospho akt ser473 d9e antibody

Human Insulin R (rDNA origin) was manufactured by Eli Lilly and Company (Indianapolis, IN). Mitochondria Isolation kit was purchased from Milteny Biotec (Auburn, CA). Protein Assay Dye Reagent Concentrate was purchased from Bio-Rad (Hercules, CA). Anti-phospho Akt (Ser473) (D9E) antibody was purchased from Cell SignalingTechnology (Danvers, MA). Anti-SOD2 antibody (9E2BD2) was purchased from Abcam (Cambridge, MA). CyDye fluorescent dyes, pH 3-7 NL and pH 3-11 NL Immoboline DryStrips gels were purchased from GE Healthcare Life Sciences (Pittsburgh, PA). Precast gradient SDS-PAGE gels were purchased from JULE, Inc. (Milford, CT). α-cyano-4-hydroxycinnamic acid was purchased from ProteoChem (Loves Park, IL). All other chemicals were purchased from Sigma-Aldrich Corporation (St. Louis, MO). Nunc brand, clear, flat bottom, 96-well microplates were purchased from Thermo Fisher Scientific (Waltham, MA). ZipTips_C18 were purchased from EMD Millipore (Billerica, MA).