Blood samples were collected from the submandibular vein once a week and 2 days after each MC injection for the mice with subcutaneous Hela tumor. Next, 100–200 µL blood was collected in K3-EDTA-coated tubes (Greiner, Kremsmünster, Austria), kept on ice, and centrifuged at 1000 g for 10 min at 4 °C. After tumor implantation, mice with subcutaneous tumors were kept in metabolic cages 8 h per day for 5 weeks. Urine samples from each mouse were collected and spun down at 1000 g for 10 min. Samples collected within the same week were pooled and extracted for analysis. Both blood and urine samples were stored at −80 °C prior to RNA isolation.
K3 edta coated tubes
Manufactured by Greiner
Sourced in Austria, United States
K3-EDTA-coated tubes are laboratory equipment used for the collection and storage of blood samples. The tubes contain the anticoagulant K3-EDTA, which prevents the blood from clotting during the collection and analysis process.
Automatically generated - may contain errors
3 protocols using k3 edta coated tubes
1
Tumor Monitoring via Blood and Urine
2
Lipid Profile and DNA Extraction from Diabetic Patients
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Three ml venous blood was collected from diabetic and ethnically matched non-diabetic individuals in K3EDTA coated tubes (Greiner Bio-One, North America Inc., North Carolina, USA). Plasma was separated and stored at -20°C for lipid profile estimation. FBS, total cholesterol (TC), triglycerides (TG), high-density lipoprotein (HDL) was analyzed using a commercial kit (Reckon Diagnostics P. Ltd, Vadodara, India). Low-density lipoprotein (LDL) was calculated using Friedewald’s (1972) formula. DNA was extracted from the whole blood using a QIAamp DNA Blood Mini Kit (Qiagen, Germany). The DNA content and purity were determined spectrophotometrically by 260/280 ratio. The integrity of DNA was checked electrophoretically using 0.8% agarose gel. The DNA was stored at -20°C until further analysis.
3
Quantitative Analysis of Lu AF64386
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The total concentration of Lu AF64386 was determined in the plasma. Trunk blood was collected from saline and PCP pretreated rats approx. 1 week after the last experimental day 40 minutes after s.c. administration of 2.5 mg/kg Lu AF64386.
Samples were collected in K3-EDTA-coated tubes (Greiner Bio-One, Kremsmünster, Austria) and centrifuged at 3300 x G for 10 minutes at 4°C. Samples were kept at -80°C until bioanalysis. Plasma samples were precipitated with acetonitrile containing a generic internal standard (Lu AE90074). Quantitative analysis was performed by ultra-performance liquid chromatography (Acquity UPLC system; Waters, Milford, MA) followed by tandem mass spectrometry detection (Waters Xevo TQS mass spectrometer; Waters, Milford, MA) using a positive-ion electrospray ionization mode. The lower limit of concentration was 1 ng/ml.
Samples were collected in K3-EDTA-coated tubes (Greiner Bio-One, Kremsmünster, Austria) and centrifuged at 3300 x G for 10 minutes at 4°C. Samples were kept at -80°C until bioanalysis. Plasma samples were precipitated with acetonitrile containing a generic internal standard (Lu AE90074). Quantitative analysis was performed by ultra-performance liquid chromatography (Acquity UPLC system; Waters, Milford, MA) followed by tandem mass spectrometry detection (Waters Xevo TQS mass spectrometer; Waters, Milford, MA) using a positive-ion electrospray ionization mode. The lower limit of concentration was 1 ng/ml.
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