Anti type 1 collagen antibody

Manufactured by Southern Biotech

Sourced in United States

The anti-type I collagen antibody is a laboratory reagent used to detect and quantify type I collagen in biological samples. It binds specifically to type I collagen, a major structural protein found in connective tissues such as skin, bone, and tendons. This antibody can be used in various immunoassay techniques to measure type I collagen levels, which can be important in research related to connective tissue biology, wound healing, and certain disease conditions.

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Lab products found in correlation

Dc protein assay kit, by Bio-Rad (2 mentions) Anti β actin antibody, by Merck Group (2 mentions) Anti α sma antibody, by Merck Group (1 mentions) Anti bax, by Cell Signaling Technology (1 mentions) Anti bcl 2, by Cell Signaling Technology (1 mentions) Anti p53 2524s, by Cell Signaling Technology (1 mentions) Anti gapdh antibody, by Cell Signaling Technology (1 mentions) 3 3 diaminobenzidine tetrahydrochloride dab, by Vector Laboratories (1 mentions) Anti collagen type 2, by Merck Group (1 mentions) Anti cd41, by Abcam (1 mentions) Prolong gold antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions) Anti met c12 antibody, by Santa Cruz Biotechnology (1 mentions) Anti ddk antibody, by OriGene (1 mentions) Alexa fluor 647 conjugated goat anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions) Aposensor cell viability assay kit, by Abcam (1 mentions) Anti β actin, by Merck Group (1 mentions) Tgf β, by R&D Systems (1 mentions) Anti met c 12, by Santa Cruz Biotechnology (1 mentions) Anti ctgf antibody, by Santa Cruz Biotechnology (1 mentions)

6 protocols using anti type 1 collagen antibody

Quantifying Renal Fibrosis Markers

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Kidney tissues (20mg) were homogenized in RIPA buffer (Sigmal-Aldrich). Concentration of protein was quantitated using the DC protein assay kit (Bio-Rad laboratories). Equal amounts of sample were subjected to electrophoresis through 4-12% Bis-Tris Gels and transferred to PVDF membranes. After blocking with 5% milk in TBST, the blots were incubated with anti-collagen type I antibody (SouthernBiotech, cat: 1310-01), anti-α-SMA antibody (Sigma, cat: A5228), FN (Abcam, cat: 2413), anti-GAPDH (CST, cat:2118) or anti-cleaved caspase 3 (CST, cat: 9664) overnight in 4°C. The blots were then washed and in cubated for 1 hour at room temperature with individual secondary antibodies accordingly. Bands were detected using an enhanced chemiluminescence detection system. The detected bands were quantified by densitometry through Image J 1.38 for windows.

Ren J., Rudemiller N.P., Wen Y., Lu X., Privratsky J.R, & Crowley S.D. (2019). The transcription factor Twist1 in the distal nephron but not in macrophages propagates aristolochic acid nephropathy.. Kidney international, 97(1), 119-129.

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Protein Expression Analysis in Kidney Tissue

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Kidney tissues were homogenized in RIPA buffer, and the protein concentrations were quantitated using the DC protein assay kit (Bio-Rad). Equal amounts of protein samples were subjected to electrophoresis with Bis-Tris Gels and transferred to PVDF membranes. The blots were blocked with 5% milk in TBST and incubated with anticollagen type I antibody (Cat: 1310-01, Southern Biotech), anti-Bax (2772S, Cell Signaling Technology), anti-Bcl2 (2870S, Cell Signaling Technology), anti-p53 (2524S, Cell Signaling Technology), anti-RIP3 (95702S, Cell Signaling Technology), and anti-GAPDH antibody (Cat: 2118, Cell Signaling Technology) overnight in 4°C. The blots were then washed and incubated with secondary antibodies for 1 hour at room temperature. Target bands were detected using ECL solution and quantified by Image J analysis.

Wen Y., Lu X., Privratsky J.R., Ren J., Ali S., Yang B., Rudemiller N.P., Zhang J., Nedospasov S.A, & Crowley S.D. (2023). TNF-α from the Proximal Nephron Exacerbates Aristolochic Acid Nephropathy. Kidney360, 5(1), 44-56.

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Chondrocyte Phenotypic Analysis via IHC and IF

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Chondrocytes cultured in D, M, DF, or MF (CCs-D, CCs-M, CCs-DF, or CCs-MF) were plated on cover slips, cultured for 2 d, fixed with 3.7% formalin in PBS, and permeabilized with 0.2% Triton X-100. For immunohistochemical (IHC) staining, anti-type I collagen antibody (Southern Biotech Associates) was applied to the cover slips at 4 °C overnight, and the results were developed with 0.1% 3,3′-diaminobenzidine tetrahydrochloride (DAB; Vector Laboratories, Burlingame, CA, USA) in PBS for 5 min. Nuclei were counterstained with hematoxylin. For immunofluorescence staining, cover slips were blocked with 20% normal goat serum and then reacted with anti-type II collagen (Millipore; cat. # MAB8887, monoclonal, clone # 6B3), anti-α-SMA (smooth muscle actin) (DAKO, Glostrup, Denmark; cat. # M0851, monoclonal, clone # 1A4), anti-CD41 (Abcam, Cambridge, MA, USA; cat. # ab63983, polyclonal), or anti-CD29 (DAKO; cat. #M0889, monoclonal, clone # K20). Fluorescein isothiocyanate–labeled goat anti-mouse IgG (Vector Laboratories) was used as a secondary antibody, and nuclei were counterstained for 5 min with 4′,6-diamidino-2-phenylindole.

Lee J., Lee J.Y., Chae B.C., Jang J., Lee E, & Son Y. (2017). Fully Dedifferentiated Chondrocytes Expanded in Specific Mesenchymal Stem Cell Growth Medium with FGF2 Obtains Mesenchymal Stem Cell Phenotype In Vitro but Retains Chondrocyte Phenotype In Vivo. Cell Transplantation, 26(10), 1673-1687.

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Smad2 Activation and Collagen Dynamics

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M10 and 10 amino acids scrambled peptides were obtained from GenScript (Piscataway, NJ), red fluorescent 5,6- carboxytetramethyl-rhodamine, succinimidyl ester (5,6-TAMRA)-conjugated M10 was purchased from BioSynthesis (Lewisville, Texas). Anti-type I collagen antibody was from Southern Biotechnology (Birmingham, AL), anti-Met (C12) antibody was from Santa Cruz Biotechnology (Santa Cruz, CA), anti-Smad2 and anti-phospho-Smad2 was from Cell Signaling Technology (Danvers, MA), anti-β-actin was from Sigma (St. Louis, MO). Alexa Fluor 647® conjugated goat anti-rabbit secondary antibody, Alexa Fluor 488^® Phalloidin, and ProLong® Gold anti-fade mountant with DAPI were obtained from Life Technologies (Grand Island, NY). Recombinant human Smad2 (NM_005901) with C-terminal MYC/DDK tag, Smad4 (NM_005359) with C-terminal MYC/DDK tag, and anti-DDK antibody were purchased from OriGene Technologies (Rockville, MD), TGFβ was from R&D Systems (Minneapolis, MN), bleomycin sulfate was from Hospira Inc. (Lake Forest, IL). ApoSENSOR^™ Cell Viability Assay Kit was obtained from BioVision (Milpitas, CA).

Atanelishvili I., Shirai Y., Akter T., Buckner T., Noguchi A., Silver R.M, & Bogatkevich G.S. (2015). M10, a Caspase Cleavage Product of the Hepatocyte Growth Factor Receptor, Interacts with Smad2 and Demonstrates Anti-Fibrotic Properties in Vitro and in Vivo. Translational research : the journal of laboratory and clinical medicine, 170, 99-111.

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Immunoblotting Analysis of MET Phosphorylation

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Cells were collected and analyzed by immunoblotting as previously described [10 (link), 25 (link)]. Phosphorylation of MET was analyzed using anti-phospho-c-Met [pYpYpY^{1230/1234/1235}], pY¹³⁴⁹, and pY¹³⁵⁶ antibodies (from Life Technologies (Miami, FL), Cell Signaling Technology (Danvers, MA), and Sigma-Aldrich (St. Louis, MO) respectively). Total MET was immunobloted using anti-MET (C12) from Santa Cruz Biotechnology (Santa Cruz, CA), anti-MET (25H2) was from Cell Signaling Technology (Danvers, MA), and pro-surfactant protein C antibody was from Seven Hills Bioreagents (Cincinnati, OH). Anti-type I collagen antibody (Southern Biotechnology, Birmingham, AL), anti-CTGF antibody (Santa Cruz Biotechnology, Santa Cruz, CA), and anti-smooth-α-actin antibody (Sigma-Aldrich, St. Louis, MO) were also used.
The phosphorylation of p42/p44 MAPK isoforms and total Erk1/2 levels were analyzed using anti-phospho-Erk1/2 and Erk1/2 antibodies in accordance with the manufacturer’s instructions (Cell Signaling Technology, Danvers, MA). Immunoblots were routinely stripped and re-blotted with anti-β-actin antibody (Sigma-Aldrich, St. Louis, MO) as a loading control.

Atanelishvili I., Shirai Y., Akter T., Noguchi A., Ash K.T., Misra S., Ghatak S., Silver R.M, & Bogatkevich G.S. (2016). D1398G Variant of MET Is Associated with Impaired Signaling of Hepatocyte Growth Factor in Alveolar Epithelial Cells and Lung Fibroblasts. PLoS ONE, 11(9), e0162357.

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Immunoprecipitation Assay for Scleroderma Lung Fibroblasts

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Cells were collected and analyzed by immunoblotting as previously described (36 (link)). Anti-α-SMA and anti-β-actin antibodies were obtained from Sigma-Aldrich (St. Louis, MO); anti-type I collagen antibody was purchased from SouthernBiotech (Birmingham, AL); anti-fibronectin antibody was obtained from Santa Cruz Biotechnology (Santa Cruz, CA) and anti-LMCD1 antibody was purchased from Novus Biologicals (Centennial, CO). For immunoprecipitation assay, scleroderma lung fibroblasts were grown to confluence on 100mm plates, kept in serum-free DMEM 4 hours, incubated with TGFβ for 24 hours, washed with ice cold PBS, collected with 1ml of ice-cold RIPA buffer, and cleared by microcentrifugation at 4°C. Next, 2μg of anti-SRF antibody (Sigma) was added, and the samples were rotated for 90min at 4°C. Immune complexes were isolated on protein G-sepharose beads (Amersham Pharmacia Biotech, Piscataway, NJ), washed with RIPA buffer, resolved by gel electrophoresis, and immunoblotted with anti-LMCD1 antibody.

Bogatkevich G.S., Atanelishvili I., Bogatkevich A.M, & Silver R.M. (2023). Increased Expression of LMCD1 in Scleroderma-Associated Interstitial Lung Disease is Critical for Profibrotic Characteristics of Lung Myofibroblasts. Arthritis & rheumatology (Hoboken, N.J.), 75(3), 438-448.

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