Anti rabbit gsk3β

Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS),_L-glutamine and penicillin/streptomycin/neomycin were obtained from GIBCO BRL/Invitrogen (Carlsbad, CA, USA). Anti-rabbit MMP-2, anti-goat MMP-9, anti-mouse Bax, anti-mouse β-actin, anti-rabbit c-myc, anti-rabbit survivin, anti-rabbit Bcl-2, anti-mouse β-catenin, anti-rabbit p53, anti-rabbit caspase-3, antibodies were purchased from Santa Cruz Biotechnology, Inc. (Heidelberg, Germany). Anti-mouse cyclin D1, anti-rabbit PARP, anti-mouse caspase-3/-9, anti-mouse CDK4, anti-rabbit GSK3β, anti-rabbit p-GSK3β, anti-rabbit p-β-catenin and anti-rabbit histone H3 antibodies were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). Protease inhibitor MG132 and GSK3β inhibitor SB216763 were purchased from Merk KGaA (Darmstadt, Germany). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Cycloheximide, was purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). CoQ₀ (Coenzyme Q₀, 2,3 dimethoxy-5-methyl-1,4 benzoquinone) were purchased from Sigma-Aldrich (St. Louis, MO). All other chemicals were of the highest grade commercially available and were supplied either by Merck or Sigma.