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Pinducer20 inducible lentiviral expression vector

Manufactured by Thermo Fisher Scientific
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The PInducer20 inducible lentiviral expression vector is a laboratory tool designed for regulated gene expression. It provides a controlled system for introducing and expressing genes of interest in target cells. The vector includes necessary elements for lentiviral packaging and delivery, as well as an inducible promoter for regulated transgene expression.

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Lab products found in correlation

Protein purification kit, by Takara Bio (2 mentions) Pcdna dest40, by Thermo Fisher Scientific (2 mentions) Gateway system, by Thermo Fisher Scientific (2 mentions) Bl21 codonplus de3 ripl, by Agilent Technologies (2 mentions) Quikchange lightning site directed mutagenesis kit, by Agilent Technologies (2 mentions)

2 protocols using pinducer20 inducible lentiviral expression vector

1

Mammalian and Bacterial Expression Vectors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mammalian expression vectors for full-length LINK-A and the deletion mutant were constructed by subcloning the gene sequences into a pCDNA3.1 (+) backbone and pInducer20 inducible lentiviral expression vector (Life Technologies). Mammalian expression of full-length TRIM71, PKA C-α, CNR2, GABR1, ADA2A, ACM4, OPRM, and mutant vectors were constructed by subcloning the corresponding gene sequences into the His-tagged expression vector (pcDNA™-DEST40) using the Gateway system (Life Technologies). Bacteria expression of full-length CNR2, GABR1, ADA2A, ACM4, OPRM, and mutant vectors was constructed by subcloning the corresponding gene sequences into the GST-tagged expression vector pGEX-5X-1. All single-point and deletion mutations were generated using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies). Recombinant proteins were expressed in the Escherichia coli strain BL21-CodonPlus (DE3)-RIPL (Agilent Technologies) and purified using the Protein Purification Kit (Clontech).
Hu Q., Ye Y., Chan L.C., Li Y., Liang K., Lin A., Egranov S.D., Zhang Y., Xia W., Gong J., Pan Y., Chatterjee S.S., Yao J., Evans K.W., Nguyen T.K., Park P.K., Liu J., Coarfa C., Donepudi S.R., Putluri V., Putluri N., Sreekumar A., Ambati C.R., Hawke D.H., Marks J.R., Gunaratne P.H., Caudle A.S., Sahin A.A., Hortobagyi G.N., Meric-Bernstam F., Chen L., Yu D., Hung M.C., Curran M.A., Han L., Lin C, & Yang L. (2019). Oncogenic LncRNA downregulates cancer cell antigen presentation and intrinsic tumor suppression. Nature immunology, 20(7), 835-851.
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2

Mammalian and Bacterial Expression Vectors

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mammalian expression vectors for full-length LINK-A and the deletion mutant were constructed by subcloning the gene sequences into a pCDNA3.1 (+) backbone and pInducer20 inducible lentiviral expression vector (Life Technologies). Mammalian expression of full-length TRIM71, PKA C-α, CNR2, GABR1, ADA2A, ACM4, OPRM, and mutant vectors were constructed by subcloning the corresponding gene sequences into the His-tagged expression vector (pcDNA™-DEST40) using the Gateway system (Life Technologies). Bacteria expression of full-length CNR2, GABR1, ADA2A, ACM4, OPRM, and mutant vectors was constructed by subcloning the corresponding gene sequences into the GST-tagged expression vector pGEX-5X-1. All single-point and deletion mutations were generated using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent Technologies). Recombinant proteins were expressed in the Escherichia coli strain BL21-CodonPlus (DE3)-RIPL (Agilent Technologies) and purified using the Protein Purification Kit (Clontech).
Hu Q., Ye Y., Chan L.C., Li Y., Liang K., Lin A., Egranov S.D., Zhang Y., Xia W., Gong J., Pan Y., Chatterjee S.S., Yao J., Evans K.W., Nguyen T.K., Park P.K., Liu J., Coarfa C., Donepudi S.R., Putluri V., Putluri N., Sreekumar A., Ambati C.R., Hawke D.H., Marks J.R., Gunaratne P.H., Caudle A.S., Sahin A.A., Hortobagyi G.N., Meric-Bernstam F., Chen L., Yu D., Hung M.C., Curran M.A., Han L., Lin C, & Yang L. (2019). Oncogenic LncRNA downregulates cancer cell antigen presentation and intrinsic tumor suppression. Nature immunology, 20(7), 835-851.
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