Compound C (CC; P5499), Palmitate (P5585), FGF21 protein (SRP6184), Minimum Essential Medium Eagle (MEM; M4526),Rosiglitazone(Ros, R2408) were purchased from Sigma- Aldrich. GW9662 (70785) was got from Cayman chemical Company (Ann Arbor, MI). 2-[N-(7-Nitrobenz- 2-oxa-1,3- diazol-4-yl)amino]-2-deoxyglucose (2-NBDG; N13195) and Lipofectamine™ 2000 transfection reagent (11668–019) were purchased from Invitrogen. Fetal bovine serum (FBS; SH30370.03) was purchased from Hyclone Laboratories. Antibodies against p-AMPK (sc-33524), AMPK (sc-74461), insulin receptor substrate-1 (IRS-1; sc-599), p-IRS-1 tyr465 (sc-3956), siRNAs for PPARγ(sc-156077),FGF21 (sc-156171) and AMPK (sc- 155985), control (sc-44230) were obtained from Santa Cruz Biotechnology. Antibodies against protein kinase B (Akt; 9272) and p-Akt (9271) were obtained from Cell Signaling Technology. Antibodies against ACTB/β-actin (TA-09) was obtained from Zhongshan Jinqiao Biotechnology Co. APL (msat-120131108, HPLC≥98%) was purchased from Chengdu MUST Bio- Technology co., LTD.
2 n 7 nitrobenz 2 oxa 1 3 diazol 4 yl amino 2 deoxyglucose 2 nbdg n13195
Manufactured by Thermo Fisher Scientific
Sourced in United States
2-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino]-2-deoxyglucose (2-NBDG; N13195) is a fluorescent dye used for the detection of glucose uptake in cells. It functions as a fluorescent glucose analog, allowing for the visualization and quantification of glucose transport and metabolism in living cells.
Automatically generated - may contain errors
Lab products found in correlation
P akt 9271, by Cell Signaling Technology (1 mentions)
Lipofectamine 2000 transfection reagent, by Thermo Fisher Scientific (1 mentions)
Bovine calf serum (bcs), by Thermo Fisher Scientific (1 mentions)
Insulin i5500, by Merck Group (1 mentions)
High glucose dulbecco s modified eagle s medium dmem, by Cytiva (1 mentions)
Foetal bovine serum (fbs), by Cytiva (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using 2 n 7 nitrobenz 2 oxa 1 3 diazol 4 yl amino 2 deoxyglucose 2 nbdg n13195
1
FGF21-mediated AMPK activation in metabolic regulation
2
Adipogenic Differentiation Regulation Protocol
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High glucose Dulbecco's Modified Eagle's Medium (DMEM) and foetal bovine serum (FBS) were purchased from Hyclone Laboratories, Inc. (Logan, UT, USA). Bovine calf serum was purchased from Gibco Life Technologies (Thermo Fisher, Carlsbad, CA, USA). DHM (SML0295), rosiglitazone (ROSI, R2408), GW9662 (M6191), PD98059 (P215), 3‐isobutyl‐1‐methylxanthine (IBMX, I7018), dexamethasone (DEX, D4902) and insulin (I5500) were obtained from Sigma‐Aldrich (St. Louis, MO, USA). 2‐[N‐(7‐nitrobenz‐2‐oxa‐1,3‐diazol‐4‐yl) amino]‐2‐deoxyglucose (2‐NBDG; N13195) was obtained from Invitrogen (Thermo Fisher, Carlsbad, CA, USA). Antibodies against PPARγ, phospho‐CDK5 (Ser159), CDK5, phospho‐ERK1/2 (Thr202/Tyr204), ERK and β‐actin were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Antibody against fatty acid‐binding protein 4 (FABP4) was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Antibody against phospho‐PPARγ (Ser273) was obtained from Biosynthesis Biotechnology Co., Ltd. (Beijing, China).
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