Two human CRC cell lines, HCT116 and SW480, were obtained from Procell (Wuhan, China) and the National Infrastructure of Cell Line Resource (NICR), respectively. HCT116 and SW480 cells were cultured in McCoy 5A (Procell, China) and IMDM (Procell, China) medium containing 10% FBS, respectively. Cells were maintained in a wet incubator with 5% CO2 at 37°C. The recombinant lentivirus used for SH3TC2 knockdown (sh-SH3TC2#1, #2) and the corresponding control lentivirus (sh-NC) were purchased from GeneChem (Shanghai, China). The overexpression plasmids for wide-type (OE-YTHDF1-WT) and mutant (OE-YTHDF1-Mut) YTHDF1 were also obtained from GeneChem (Shanghai, China). The reagents HiTransG A (GeneChem, China) and Lipo3000 (Thermo, USA) were utilized to transfect lentiviruses and plasmids into CRC cells, respectively.
Mccoy 5a
Manufactured by Procell
Sourced in China
The McCoy 5A is a laboratory instrument designed for basic analytical measurements. It is a compact and versatile device capable of performing various tasks in a laboratory setting.
Automatically generated - may contain errors
2 protocols using mccoy 5a
1
Knockdown and Overexpression of SH3TC2 and YTHDF1 in CRC Cells
2
CEBPB Knockdown in HCT116 CRC Cells
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The human CRC cell line HCT116 was obtained from Procell (Wuhan, China) and the National Infrastructure of Cell Line Resource (NICR), respectively. HCT116 cells were cultured in McCoy 5A (Procell, China) and IMDM (Procell, China) medium containing 10% FBS, respectively. Cells were maintained in a wet incubator with 5% CO2 at 37°C. The recombinant lentivirus used for CEBPB knockdown (sh‐CEBPB#1, #2) and the corresponding control lentivirus (sh‐NC) were purchased from GeneChem (Shanghai, China). The reagent HiTransG A (GeneChem, China) was utilized to transfect lentiviruses into CRC cells.
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