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Akt aho1112

Manufactured by Thermo Fisher Scientific
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The AKT (AHO1112) is a versatile laboratory instrument designed for a variety of analytical applications. It functions as a precision centrifuge, providing reliable and consistent performance for sample preparation and separation. The AKT is a compact and durable device, engineered to meet the needs of modern research laboratories.

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Lab products found in correlation

Hek293t cell line, by American Type Culture Collection (2 mentions) Hek293t cell, by American Type Culture Collection (2 mentions) Anti mouse 7076, by Cell Signaling Technology (2 mentions) Dulbecco modified eagle medium (dmem), by American Type Culture Collection (2 mentions) Cdc2 9112, by Cell Signaling Technology (2 mentions) Ha 7392 antibodies, by Santa Cruz Biotechnology (2 mentions) Flag antibodies a2220, by Merck Group (2 mentions) Gfp 33 2600, by Thermo Fisher Scientific (2 mentions) P21 33 7000, by Thermo Fisher Scientific (2 mentions) P53 13 4100, by Thermo Fisher Scientific (2 mentions) Phospho akt s473 44 609g, by Thermo Fisher Scientific (2 mentions) Beta actin 8457, by Cell Signaling Technology (2 mentions) Roswell park memorial institute (rpmi), by American Type Culture Collection (2 mentions) Cyclin b1 4138, by Cell Signaling Technology (2 mentions) Cyclin a2 bf683, by Cell Signaling Technology (2 mentions)

2 protocols using akt aho1112

1

Cell Line Characterization and Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293T cell line was obtained from the American Type Culture Collection (ATCC; Rockville, MD). RWP1, Mia Paca-2 and Panc02.03 cell lines were kindly provided by Dr. Jason Moffat. HEK293T cells and the pancreatic cancer RWP1, Mia Paca-2 cell lines were maintained in DMEM (ATCC) supplemented with 10% FBS and 1% pen/strep/glutamine, and the appropriate selection antibiotics when required. Panc02.03 cells were maintained in RPMI (ATCC) supplemented with 20% FBS and 1% pen/strep/glutamine, and the appropriate selection antibiotics when required. Antibodies were obtained from the following commercial sources: HA (7392) antibodies (1:2000) were purchased from Santa Cruz. Flag antibodies (A2220) (1:1000) were purchased from Sigma. GFP (33-2600) (1:2000), p21 (33-7000) (1:1000), p53 (13-4100) (1:1000), AKT (AHO1112) (1:1000), phospho-AKT (S473) (44-609G) (1:1000) antibodies were purchased from Invitrogen. Cyclin B1 (4138) (1:1000), cyclin A2 (BF683) (1:2000), cdc2 (9112) (1:1000), beta-actin (8457) (1:1000), anti-mouse (7076) (1:2500) and anti-rabbit (1:2500) HRP-linked (7074) antibodies were purchased from Cell Signaling.
Nim S., Jeon J., Corbi-Verge C., Seo M.H., Ivarsson Y., Moffat J., Tarasova N, & Kim P.M. (2016). Pooled screening for anti-proliferative inhibitors of protein-protein interactions. Nature chemical biology, 12(4), 275-281.
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2

Cell Line Characterization and Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293T cell line was obtained from the American Type Culture Collection (ATCC; Rockville, MD). RWP1, Mia Paca-2 and Panc02.03 cell lines were kindly provided by Dr. Jason Moffat. HEK293T cells and the pancreatic cancer RWP1, Mia Paca-2 cell lines were maintained in DMEM (ATCC) supplemented with 10% FBS and 1% pen/strep/glutamine, and the appropriate selection antibiotics when required. Panc02.03 cells were maintained in RPMI (ATCC) supplemented with 20% FBS and 1% pen/strep/glutamine, and the appropriate selection antibiotics when required. Antibodies were obtained from the following commercial sources: HA (7392) antibodies (1:2000) were purchased from Santa Cruz. Flag antibodies (A2220) (1:1000) were purchased from Sigma. GFP (33-2600) (1:2000), p21 (33-7000) (1:1000), p53 (13-4100) (1:1000), AKT (AHO1112) (1:1000), phospho-AKT (S473) (44-609G) (1:1000) antibodies were purchased from Invitrogen. Cyclin B1 (4138) (1:1000), cyclin A2 (BF683) (1:2000), cdc2 (9112) (1:1000), beta-actin (8457) (1:1000), anti-mouse (7076) (1:2500) and anti-rabbit (1:2500) HRP-linked (7074) antibodies were purchased from Cell Signaling.
Nim S., Jeon J., Corbi-Verge C., Seo M.H., Ivarsson Y., Moffat J., Tarasova N, & Kim P.M. (2016). Pooled screening for anti-proliferative inhibitors of protein-protein interactions. Nature chemical biology, 12(4), 275-281.
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