DNA and RNA oligonucleotides (S5 and S6 Tables) were purchased from Beijing TSINGKE Biological Technology and Shanghai GenePharma Corporation respectively. Antibodies used in this study were listed as follows: anti-ACE2 (Abcam, ab108252), anti-AXL (ABclone, A17874), anti-FLAG (ABclone, AE004), anti-FURIN (Abcam, ab183495), anti-GAPDH (Proteintech, 60004–1), anti-HA (CST, 3724S) and anti-TMPRSS2 (Abcam, ab280567).
Anti flag
Manufactured by Abclone
Sourced in Germany
Anti-FLAG is a laboratory reagent used for the detection and purification of proteins with a FLAG tag. The FLAG tag is a short peptide sequence that can be genetically fused to a target protein, allowing for its identification and isolation using Anti-FLAG antibodies or affinity resins.
Automatically generated - may contain errors
2 protocols using anti flag
1
Antibodies for COVID-19 research
2
Characterization of Fish Immune Responses
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The MG132, 3-Methyladenine (3-MA), and ammonium chloride (NH4Cl) were from MCE (New Jersey, USA). The poly(I:C), COPP, and 3% H2O2 were purchased from Merck (Darmstadt, Germany).
Mouse anti-HA (1:5000), anti-Flag (1:5000), anti-His (1:3000), anti-GFP (1:5000), and rabbit anti-β-actin (1:10000), anti-HO-1 (1:1000), anti-IRF3 (1:1000), anti-IRF7 (1:1000), anti-IFN-α (1:500), and anti-LC3 (1:2000), were ordered from ABclone (Boston, USA). Secondary antibodies against mouse (1:5000) and rabbit (1:5000) were provided by Abcam (Cambridge, UK). Rabbit polyclonal anti-Pimephales promelas IFN antiserum was prepared by immunization of animals with prokaryotic-expressed proteins previously made in our laboratory. The rabbit anti-SVCV-G and mouse anti-Grass Carp HO-1 antiserum were kindly gifted by Prof. Xueqin Liu (Huazhong Agricultural University) and Prof. Zhendong Qin (Zhongkai University of Agriculture and Engineering), respectively.
Mouse anti-HA (1:5000), anti-Flag (1:5000), anti-His (1:3000), anti-GFP (1:5000), and rabbit anti-β-actin (1:10000), anti-HO-1 (1:1000), anti-IRF3 (1:1000), anti-IRF7 (1:1000), anti-IFN-α (1:500), and anti-LC3 (1:2000), were ordered from ABclone (Boston, USA). Secondary antibodies against mouse (1:5000) and rabbit (1:5000) were provided by Abcam (Cambridge, UK). Rabbit polyclonal anti-Pimephales promelas IFN antiserum was prepared by immunization of animals with prokaryotic-expressed proteins previously made in our laboratory. The rabbit anti-SVCV-G and mouse anti-Grass Carp HO-1 antiserum were kindly gifted by Prof. Xueqin Liu (Huazhong Agricultural University) and Prof. Zhendong Qin (Zhongkai University of Agriculture and Engineering), respectively.
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