Anti hnrnpa3 antibody

Manufactured by Proteintech

The Anti-hnRNPA3 antibody is a laboratory tool used for the detection and study of the hnRNPA3 protein. hnRNPA3 is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family, which are involved in various aspects of mRNA processing and regulation.

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Lab products found in correlation

Vectastain elite abc universal plus kit, by Vector Laboratories (2 mentions) Orca flash 4.0 camera, by Hamamatsu Photonics (2 mentions) 3 3 diaminobenzidine (dab), by Merck Group (2 mentions) Bx57 microscope, by Olympus (2 mentions) Hrp conjugated anti rabbit igg antibody, by Vector Laboratories (1 mentions)

Close spelling variants of this product

HnRNPA3 (3 citations) Anti-TM (2 citations)

2 protocols using anti hnrnpa3 antibody

hnRNPA3 Immunohistochemistry in FUS-TG Mice

Cited 1 time

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Spinal cord sections (8 μm thick) from wild-type and 4-month old symptomatic FUS-TG mice (Shelkovnikova et al., 2013a (link)) were used. After rehydration, sections were subjected to microwave antigen retrieval in sodium citrate buffer (pH 6.0) and blocked using 10% goat serum in PBS/T. Sections were incubated with the primary anti-hnRNPA3 antibody (Proteintech, 25142–1-AP) overnight at 4 °C and secondary HRP-conjugated anti-rabbit IgG antibody (Vector Laboratories) for 1.5 h at RT. Signal was detected using Vectastain® Elite ABC Universal Plus Kit (Vector Laboratories) and 3,3′-diaminobenzidine (DAB, Sigma). Images were taken using BX57 microscope (Olympus) and ORCA-Flash 4.0 camera (Hamamatsu).

An H., Litscher G., Watanabe N., Wei W., Hashimoto T., Iwatsubo T., Buchman V.L, & Shelkovnikova T.A. (2022). ALS-linked cytoplasmic FUS assemblies are compositionally different from physiological stress granules and sequester hnRNPA3, a novel modifier of FUS toxicity. Neurobiology of Disease, 162, 105585.

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Immunohistochemical Analysis of hnRNPA3 in FUS-TG Mouse Spinal Cord

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Spinal cord sections (8 µm thick) from wild-type and 4-month old symptomatic FUS-TG mice (Shelkovnikova et al., 2013a) were used. After rehydration, sections were subjected to microwave antigen retrieval in sodium citrate buffer (pH 6.0) and blocked using 10% goat serum in PBS/T. Sections were incubated with the primary anti-hnRNPA3 antibody (Proteintech, 25142-1-AP) overnight at 4 C and secondary HRP-conjugated anti-rabbit IgG antibody (Vector Laboratories) for 1.5 h at RT. Signal was detected using Vectastain® Elite ABC Universal Plus Kit (Vector Laboratories) and 3,3′-diaminobenzidine (DAB, Sigma). Images were taken using BX57 microscope (Olympus) and ORCA-Flash 4.0 camera (Hamamatsu).

An H., Litscher G., Wei W., Watanabe N., Hashimoto T., Iwatsubo T., Buchman V.L., & Shelkovnikova T.A. (2021). Compositional analysis of ALS-linked stress granule-like structures reveals factors and cellular pathways dysregulated by mutant FUS under stress.

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