CuCl2·2H2O, sodium citrate, Na2S·9H2O, tetraethyl orthosilicate (TEOS), triethanolamine (TEA), hydrochloric acid (HCl, 37%), ethanol, and ammonia solution (25–28%) were purchased from Sinopharm Chemical Reagent Co. (Shanghai, China). Disulfiram (DSF), cetyltrimethylammonium chloride (CTAC, 25 wt%), 3-mercaptopropyltrimethoxysilane (MPTMS), and 2, 2′-dipyridyl disulfide (DPDS) were obtained from Sigma-Aldrich. Bis(3-triethoxysilylproyl)disulfide (BTES) was bought from Lark Chemical Technology Co., Ltd. HS-PEG-SH was purchased from Shanghai Yarebio Technology Co., Ltd. Calcein-AM/PI double staining kit was purchased from Beijing Solarbio Science & technology Co., Ltd. Cell counting kit-8 (CCK-8) was purchased from Dojindo Molecular Technologies. Annexin V-FITC/PI apoptosis detection kit was purchased from Vazyme (Nanjing, China). Cell culture medium 1640, trypsin–EDTA and fetal bovine serum (FBS) were provided by Gibco-BRL (Burlington, Canada). H&E and Ki-67 cell proliferation kit were purchased from Beyotime Biotechnology Co. (Haimen, China). The primary antibody rabbit anti-NPL4 antibody was bought from Abcam. The second antibody anti-rabbit IgG (horseradish peroxidase (HRP)-linked antibody) was bought from Cell Signaling Technology, Inc. All chemicals were used as received without further purification, and their aqueous solutions were prepared using deionized water.
Cell culture medium 1640
Manufactured by Thermo Fisher Scientific
Sourced in Canada, United States
Cell culture medium 1640 is a common, widely used medium formulation for the in vitro cultivation of a variety of cell types, including mammalian cells. It provides the necessary nutrients and components to support cell growth and proliferation in a controlled laboratory environment.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Calcein am pi double staining kit, by Solarbio (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
3 mercaptopropyl trimethoxysilane, by Merck Group (1 mentions)
2 2 dipyridyl disulfide, by Merck Group (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Vazyme (1 mentions)
Ecl plus kit, by Thermo Fisher Scientific (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Rabbit polyclonal anti human p65, by Cell Signaling Technology (1 mentions)
P erk1 2, by Cell Signaling Technology (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
C fos, by Cell Signaling Technology (1 mentions)
Human cd34 apc, by BD (1 mentions)
Cremophor el, by Merck Group (1 mentions)
C myc, by Cell Signaling Technology (1 mentions)
Caspase 9, by Cell Signaling Technology (1 mentions)
P c jun, by Cell Signaling Technology (1 mentions)
C jun, by Cell Signaling Technology (1 mentions)
Annexin 5 fluorescein isothiocyanate fitc, by BD (1 mentions)
2 protocols using cell culture medium 1640
1
Synthesis and Characterization of Multifunctional Nanoparticles
2
Apoptosis Evaluation in Cell Cultures
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Cell culture medium (1640) and foetal bovine serum were purchased from Gibco (NY, USA). H4434 culture medium was purchased from stem cell. MTT, cremophor EL, and DMSO were purchased from Sigma Chemical Company (St. Louis, MA, USA). Cell lysis buffer was purchased from Beyotime Institute of Biotechnology (Beijing, China). AnnexinV-fluorescein isothiocyanate (FITC) and propidium iodide (PI) apoptosis detection kit, human CD34-APC, and human CD38-PE.cy7 antibody were purchased from BD (BD, USA). Rabbit polyclonal anti-human p65, XIAP, Bax, Bcl-2, JNK, p-JNK, ERK1/2, p-ERK1/2, p38, p-p38, c-Jun, p-c-Jun, c-Fos, c-Myc, PARP, caspase-3, caspase-9, and β-actin antibodies were purchased from Cell Signaling Technology (Beverly, MA, USA). ECL-Plus Kit was purchased from Thermo Scientific (Rockford, IL, USA). Kunming mice and NOD/SCID mice were purchased from Chinese Academy of Sciences (Shanghai, China).
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