Ab253219

Manufactured by Abcam

Sourced in United States

Ab253219 is a laboratory product manufactured by Abcam. It is a tool for research purposes, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. Therefore, a more detailed description is not available.

Automatically generated - may contain errors

Lab products found in correlation

Ab102531, by Abcam (1 mentions) Ab253217, by Abcam (1 mentions) Ab102523, by Abcam (1 mentions) Emmpo, by Thermo Fisher Scientific (1 mentions) Ab279417, by Abcam (1 mentions) Ab282874, by Abcam (1 mentions) Ab272204, by Abcam (1 mentions) Ab208348, by Abcam (1 mentions)

3 protocols using ab253219

Biomarker Quantification Protocol

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The amylase (#ab102523, Abcam), myeloperoxidase level (#EMMPO, Thermo Fisher Scientific), trypsin (#ab102531, Abcam), 4-HNE (#STA-838, Cell Biolabs), 8-OHG (#K4160, BioVision), IL-6 (#EH2IL6 and #KMC0061, Thermo Fisher Scientific), and NOS2 (#ab253219 and #ab253217, Abcam) concentrations or activity in the samples were measured using ELISA kits.

Dai E., Han L., Liu J., Xie Y., Zeh H.J., Kang R., Bai L, & Tang D. (2020). Ferroptotic damage promotes pancreatic tumorigenesis through a TMEM173/STING-dependent DNA sensor pathway. Nature Communications, 11, 6339.

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Quantifying Inflammatory Markers in FACS-Isolated Cells

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Total protein was extracted from FACS-isolated cells to be used for ELISA analysis with specific kits for mouse NLRP3 (ab279417; Abcam, Cambridge, MA, USA), nitric oxide synthase (iNOS, ab253219; Abcam), tumor necrosis factor alpha (TNFα, ab208348; Abcam), interferon gamma (IFNγ, ab282874; Abcam), CD163 (ab272204; Abcam) and proliferating cell nuclear antigen (PCNA, ab196270; Abcam).

Peng Y, & Gao Y. (2022). NLRP3+ macrophages aggravate inflammatory cystitis in diabetes. Frontiers in Immunology, 13, 1057746.

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Pancreatic Biomarkers and Inflammatory Cytokines

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Serum amylase and lipase levels were used as markers of pancreatitis and were measured with an enzyme-linked immunosorbent assays (ELISA) Kit (ab137969, Abcam, Cambridge, UK; D799801, Sangon Biotech (Shanghai, China)). TNF-α, IL-1β and IL-10 levels in serum samples or tissue lysates were also determined using ELISA Kits (D711045, D711047, and D711114, respectively) from Sangon Biotech and iNOS enzymatic concentrations were evaluated using a kit from Abcam (ab253219, Abcam).

Chen W., Wang Y., Xia W., Zhang J, & Zhao Y. (2022). Neddylation-mediated degradation of hnRNPA2B1 contributes to hypertriglyceridemia pancreatitis. Cell Death & Disease, 13(10), 863.

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