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2 protocols using anti γ h2ax 613402 clone 2f3

1

Immunofluorescence Assay for DNA Damage Response

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Primary antibodies: anti-γ-H2AX (613402 clone 2F3, Biolegend); anti-TRF1 (Karlseder lab); anti-TRF2 (Karlseder lab); anti-53BP1 (H-300) (sc-22760, Santa Cruz); anti-LIG4 (ab80514, Abcam); anti-pericentrin (ab4448, Abcam); anti-MPM-2 (05-368MG, Millipore); anti-γ-tubulin (T6557, Sigma-Aldrich); anti-H3S10P (D2C8) (3377, Cell Signaling); anti-GAPDH (A300-641A, Bethyl); anti-p53 (sc-126, Santa Cruz).
Secondary antibodies: HPR-linked anti-mouse or anti-rabbit (NXA931 or NA934V; GE Healthcare); Alexa-488-conjugated anti-rabbit (Invitrogen); Alexa-594-conjugated anti-mouse (Invitrogen).
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2

Immunofluorescence Assay for DNA Damage Response

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies: anti-γ-H2AX (613402 clone 2F3, Biolegend); anti-TRF1 (Karlseder lab); anti-TRF2 (Karlseder lab); anti-53BP1 (H-300) (sc-22760, Santa Cruz); anti-LIG4 (ab80514, Abcam); anti-pericentrin (ab4448, Abcam); anti-MPM-2 (05-368MG, Millipore); anti-γ-tubulin (T6557, Sigma-Aldrich); anti-H3S10P (D2C8) (3377, Cell Signaling); anti-GAPDH (A300-641A, Bethyl); anti-p53 (sc-126, Santa Cruz).
Secondary antibodies: HPR-linked anti-mouse or anti-rabbit (NXA931 or NA934V; GE Healthcare); Alexa-488-conjugated anti-rabbit (Invitrogen); Alexa-594-conjugated anti-mouse (Invitrogen).
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