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3 protocols using boric acid

1

Synthesis of Gold Nanoparticles

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Chloroauric acid (HAuCl4·3H2O), trisodium citrate dihydrate (Na3C6H5O7·2H2O) and tris-(2-carboxyethyl)-phosphine (TCEP) were bought from Sigma (St. Louis, MO, USA). Tris base, boric acid, EDTA disodium salt, dithiothreitol (DTT), and the DNA and RNA sequences (Table S3) were supplied by Sangon Biotechnology Co., Ltd. (Shanghai, China). 5 nm and 25 nm of gold nanoparticles (AuNPs) were synthesized by Nanjing Xianfeng Nanomaterials Technology Co., Ltd. (Nanjing, China). Some analytically pure chemical reagents were not required to be purified. All solutions were prepared from 0.1% DEPC water (DEPC-H2O).
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2

Reagents for Microbial Analysis

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Plate count agar (PCA) and iron agar medium were purchased from Qingdao Hope Bio-Technology Co., Ltd. (Qingdao, Shandong, China). Magnesium oxide, boric acid, sodium chloride, and 2-thiobarbituric acid were purchased from Sangon Biotech (Shanghai) Co., Ltd. (Shanghai, China). Other agents of analytic grade were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China).
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3

Extraction and Analysis of Fungal Metabolites

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Ultrapure water was produced by the TW-D24UV system of Millitrack (Haryana, India). The yeast nitrogen base without amino acids (YNB), yeast extract peptone dextrose (YPD), chitosan, and chloramphenicol were purchased from Sangon Biotech (Shanghai, China). Chinese Baijiu (52%, v/v) was purchased from a supermarket (Dalian, China). EC was procured from Sigma-Aldrich Co. (St. Louis, MO, USA). TES buffer (300 mmol/L NaCl, 50 mmol/L Tris-HCl, 25 mmol/L EDTA, 0.2% (v/v) SDS, 2 mg/mL proteinase K, pH 8.0) was purchased from TransGen Biotech (Beijing, China). Tris-saturated phenol solution (pH 8.0), absolute ethanol, sodium acetate (pH 5.3), CaCl2, boric acid, and chloroform-isoamyl alcohol were purchased from Sangon Biotech (Shanghai, China). Glucose, glycerol, sucrose, mannose, and sorbose were purchased from Damao Co. (Tianjin, China). 4-methyl-1-pentanol was purchased from Aladdin Biotech (Shanghai, China).
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