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Taqman fast universal pcr mix

Manufactured by Thermo Fisher Scientific
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TaqMan Fast Universal PCR Mix is a ready-to-use solution designed for fast and efficient quantitative real-time PCR (qPCR) experiments. It contains all the necessary components, including a thermostable DNA polymerase, dNTPs, and optimized buffer system, to amplify and detect target DNA sequences.

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Lab products found in correlation

Taqman assay, by Thermo Fisher Scientific (2 mentions) 7500 fast real time pcr system, by Thermo Fisher Scientific (2 mentions) Steponeplus machine, by Thermo Fisher Scientific (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Superscript 2, by Thermo Fisher Scientific (1 mentions) Taqman mirna reverse transcription kit, by Thermo Fisher Scientific (1 mentions) Taqman mirna assay, by Thermo Fisher Scientific (1 mentions) Stepone software, by Thermo Fisher Scientific (1 mentions) Taqman primer, by Thermo Fisher Scientific (1 mentions) Steponeplus real time pcr system, by Thermo Fisher Scientific (1 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

TaqMan Fast Universal PCR Master Mix (2×) ABI TaqMan Fast Universal PCR Master Mix TaqMan (R) Fast Universal PCR Master Mix TaqMan Fast Universal PCR Master Mix without AmpErase UNG TaqMan Universal Fast PCR master mix reagents TaqMan™ Fast Universal PCR Master Mix (2X) no AMPERASE™ UNG TaqMan(R) Fast Universal PCR Master Mix for TaqMan assays TaqMan Fast Universal PCR Master Mix kit TaqMan® Fast Universal PCR Master Mix for TaqMan assays TaqMan Fast Universal PCR Master Mix (2X)

5 protocols using taqman fast universal pcr mix

1

Analyzing Parkinson's Disease Gene in Mice

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Midbrain tissue from C57BL/6 and BALBc Grx1−/− mice and age- and sex-matched controls (when available) was homogenized in 1mL TRIzol (Life Technologies) using a Teflon and glass mortar and pestle. RNA was isolated according to manufacturer’s instructions. Resulting RNA was converted to cDNA using SuperScriptII (Invitrogen) according to manufacturer’s instructions. Park7 (DJ-1) expression (probe Mm00498538_m1) was analyzed using TaqMan Fast Universal PCR mix (Life Technologies) on the StepOnePlus machine (Life Technologies) using StepOne v2 software. Park7 expression was normalized to Gapdh (probe Mm99999915_g1) expression as an internal control.
Johnson W.M., Golczak M., Choe K., Curran P.L., Miller O.G., Yao C., Wang W., Lin J., Milkovic N.M., Ray A., Ravindranath V., Zhu X., Wilson M.A., Wilson-Delfosse A.L., Chen S.G, & Mieyal J.J. (2016). Regulation of DJ-1 by glutaredoxin 1 in vivo – implications for Parkinson’s disease. Biochemistry, 55(32), 4519-4532.
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2

Profiling Mature miRNA Expressions

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For the screening study 500ng total RNA from each specimen was reverse transcribed by Megaplex Primer Pool Human Set v2.0 A+ B (Life Technologies), using TaqMan® miRNA Reverse Transcription kit as suggested by the manufacturer. cDNA samples of individual patients were analyzed by TaqMan® Array Human MicroRNA Card Set v2.0 A+B. For validation of differential expression, the relative expression of mature miRNAs was quantified by miRNA-specific TaqMan miRNA Assays (Life Technologies). miRNA-specific reverse transcription was carried out using TaqMan miRNA Reverse Transcription Kit (Life Technologies) using 500 ng template total RNA. RT-qPCR reactions were performed with TaqMan Fast Universal PCR Mix (Life Technologies), as recommended by the manufacturer. Ectopic and endogenous miRNA levels were normalized against RNU48, and relative expression was calculated by the ΔΔCt method.
Lichner Z., Saleh C., Subramaniam V., Seivwright A., Prud'homme G.J, & Yousef G.M. (2014). miR-17 inhibition enhances the formation of kidney cancer spheres with stem cell/tumor initiating cell properties. Oncotarget, 6(8), 5567-5581.
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3

Quantifying SNCA Expression in Brain Cortices

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Total RNA samples were isolated from brain cortices using mirVana miRNA Isolation Kit (AM1561), and RNA concentrations were determined by Nanodrop. The samples were converted into cDNA using a high‐capacity cDNA reverse transcription kit (#4368813; Applied Biosystems, Foster City, CA). Quantitative polymerase chain reaction (qPCR) was performed using the TaqMan Fast Universal PCR Mix (#4352042, Applied Biosystems) and the appropriate TaqMan primers for human SNCA (Hs00240906_m1, Applied Biosystems). qPCR reactions were run in an StepOnePlus Real‐Time PCR system, and the delta‐delta treshold cycles (ΔΔCt) calculations were made using StepOne software (Applied Biosystems).
Nuber S., Nam A.Y., Rajsombath M.M., Cirka H., Hronowski X., Wang J., Hodgetts K., Kalinichenko L.S., Müller C.P., Lambrecht V., Winkler J., Weihofen A., Imberdis T., Dettmer U., Fanning S, & Selkoe D.J. (2020). A Stearoyl–Coenzyme A Desaturase Inhibitor Prevents Multiple Parkinson Disease Phenotypes in α‐Synuclein Mice. Annals of Neurology, 89(1), 74-90.
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4

Quantitative Real-Time PCR Analysis

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, where ΔΔCT = ΔCt target mRNA -ΔCt reference mRNA (GAPDH, housekeeping gene) in the same sample. PCR experiments were performed with the 7500 Fast Real Time PCR System (Applied Biosystems), according to the manufacturer's recommendations. All reactions were performed using TaqMan Fast Universal PCR Mix (Applied Biosystems) and TaqMan Assays (Applied Biosystems) probes (see Table 2).
Kyriatzis G., Bernard A., Bôle A., Pflieger G., Chalas P., Masse M., Lécorché P., Jacquot G., Ferhat L, & Khrestchatisky M. (2021). Neurotensin receptor 2 is induced in astrocytes and brain endothelial cells in relation to neuroinflammation following pilocarpine-induced seizures in rats. Glia, 69(11).
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5

Quantitative Real-Time PCR Analysis

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, where ΔΔCT = ΔCt target mRNA -ΔCt reference mRNA (GAPDH, housekeeping gene) in the same sample. PCR experiments were performed with the 7500 Fast Real Time PCR System (Applied Biosystems), according to the manufacturer's recommendations. All reactions were performed using TaqMan Fast Universal PCR Mix (Applied Biosystems) and TaqMan Assays (Applied Biosystems) probes (see Table 2).
Kyriatzis G., Anne B., Bôle A., Guillaume P., Chalas P., Masse M., Lecorché P., Jacquot G., Ferhat L., & Khrestchatisky M. (2020). Neurotensin receptor 2 is induced in astrocytes and brain endothelial cells in relation to status epilepticus and neuroinflammation following pilocarpine administration in rats.
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