Si yap 2

Four human cervical cancer cell lines (HeLa, C33A, CaSki and ME180) from the American Type Culture Collection (ATCC, USA) were cultured in Minimum Essential Medium, Eagle's Minimum Essential Medium, RPMI-1640 Medium, and McCoy's 5a (Gibco/Thermo Fisher Scientific, USA), respectively, with 10% fetal bovine serum (FBS, Gibco) at 37 • C in a humidified atmosphere of 5% CO 2 .
To knockdown the expression of YAP in vitro, si-NC, si-YAP#1, or si-YAP#2 were purchased from GenePharma (China), and transfected into ME180, CaSki, HeLa, C33A cells by using Lipofectamine 3000 (Thermo Fisher Scientific, USA). Besides, si-NC (negative control), si-ARID1A#1, or si-ARID1A#2 (GenePharma; China) was transfected into ME180 and HeLa cells by Lipofectamine 3000. Similarly, the pcDNA3 (vector control) and pcDNA-ARID1A plasmids were also transfected into CaSki and C33A cells by using Lipofectamine 3000. After transfection, cells were treated with 0.625, 1.25, 2.5, 5 or 10 µM of verteporfin for 48 h and cell viability was detected with MTT assay.

The sense and antisense sequences of the siRNAs (GenePharma, Suzhou, China) used were as follows: si-YAP-1: 5′-GCAUCUUCGACAGUCUUCUTT-3′, 5′-AGAAGACUGUCGAAGAUGCTT-3′; si-YAP-2: 5′-GGUCAGAGAUACUUCUUAATT-3′, 5′-UUAAGAAGUAUCUCUGACCTT-3′; si-YAP-3: 5′-GGUGAUACUAUCAACCAAATT-3′, 5′-UUUGGUUGAUAGUAUCACCTT-3′; si-TAZ: 5′-AGGUACUUCCUCAAUCACATT-3′, 5′-UGUGAUUGAGGAAGUACCUTT-3′. si-TEAD1: 5′-GGAUCAGACUGCAAAGGAUTT-3′, 5′-AUCCUUUGCAGUCUGAUCCTT-3′; si-p73: 5′-CCCAAGGGUUACAGAGCAUTT-3′, 5′-AUGCUCUGUAACCCUUGGGTT-3′ and the negative control (si-NC): 5′-UUCUCCGAACGUGUCACGUTT-3′, 5′-ACGUGACACGUUCGGAGAATT-3′. Cells were transfected with 60 nM siRNA using Lipofectamine 2000 and incubated for 8 h; then, the transfection medium was replaced with fresh culture medium. The cells were subjected to subsequent experiments 72 h after transfection.