Mabs1158 for wb

Antibodies against PTPRT (MABS1158 for WB and HPA017336 for IHC) and Flag (F3165) were from Sigma; pSTAT3 (Y705) (AP0070), ADAM10 (A10438) and ADAM17 ( A0821) antibodies were from ABclonal; antibodies against STAT3 (ab68153), a-tubulin (ab7291), β-actin (ab8227), Presenlin-1 (ab15456) and Map2 (ab32454 and ab11267) were obtained from Abcam; antibodies against cleaved-Caspase3 (#9661) , a-tubulin: (#3873S), histon3 (#9715) were purchased from Cell Signaling Tech; antibodies against the 6E10 (SIG-393200) antibody were from Covance. The secondary antibodies used for immunocytochemistry were as follows: chicken anti-mouse or rabbit Alexa 488; donkey anti-mouse or rabbit Alexa 594 (Invitrogen, Eugene, OR); all were used at a dilution of 1:500. ProLong™ Diamond Antifade Mountant with DAPI (Thermo Fisher Scienti c, P36962) was used as a nuclear counterstain at 1 µg/ml. DAPT (γ-secretase inhibitor, D5942), Thio avine S (T1892), Anti-Digoxigenin-AP (Fab fragments, 11093274910), and Phorbol 12-myristate 13-acetate (PMA, p8139) were ordered from Sigma-Aldrich.

Antibodies against PTPRT (MABS1158 for WB and HPA017336 for IHC) and Flag (F3165) were from Sigma; pSTAT3 (Y705) (AP0070), ADAM10 (A10438) and ADAM17 ( A0821) antibodies were from ABclonal; antibodies against A42 (ab201061), STAT3 (ab68153), -tubulin (ab7291), β-actin (ab8227), Presenlin-1 (ab15456), VAMP2 (ab3347) and Map2 (ab32454 and ab11267) were obtained from Abcam; antibodies against cleaved-Caspase3 (#9661) , a-tubulin: (#3873S), histon3 (#9715) were purchased from Cell Signaling Tech; antibody against the A plaque 6E10 (SIG-393200) antibody were from Covance; antibody against oligomeric A MOAB-2 (NBP2-13075) and biotin-labeled MOAB-2 (NBP2-13075B) from Novus Biologicals. The secondary antibodies used for immunocytochemistry were as follows: chicken anti-mouse or rabbit Alexa 488; donkey anti-mouse or rabbit Alexa 594 (Invitrogen, Eugene, OR); all were used at a dilution of 1:500. ProLong™ Diamond Antifade Mountant with DAPI (Thermo Fisher Scientific, P36962) was used as a nuclear counterstain at 1 µg/ml. DAPT (γ-secretase inhibitor, D5942), Thioflavine S (T1892), Anti-Digoxigenin-AP (Fab fragments, 11093274910), and Phorbol 12myristate 13-acetate (PMA, p8139) were ordered from Sigma-Aldrich.

Antibodies against PTPRT (MABS1158 for WB and HPA017336 for IHC) and Flag (F3165) were from Sigma; pSTAT3 (Y705) (AP0070), ADAM10 (A10438) and ADAM17 ( A0821) antibodies were from ABclonal; antibodies against Ab 42 (ab201061), STAT3 (ab68153), α-tubulin (ab7291), β-actin (ab8227), Presenlin-1 (ab15456), VAMP2 (ab3347) and Map2 (ab32454 and ab11267) were obtained from Abcam; antibodies against cleaved-Caspase3 (#9661), a-tubulin: (#3873S), histon3 (#9715) were purchased from Cell Signaling Tech; antibody against the Ab plaque 6E10 (SIG-393200) antibody were from Covance; antibody against oligomeric Ab MOAB-2 (NBP2-13075) and biotin-labeled MOAB-2 (NBP2-13075B) from Novus Biologicals. The secondary antibodies used for immunocytochemistry were as follows: chicken anti-mouse or rabbit Alexa 488; donkey anti-mouse or rabbit Alexa 594 (Invitrogen, Eugene, OR); all were used at a dilution of 1:500. ProLong™ Diamond Antifade Mountant with DAPI (Thermo Fisher Scienti c, P36962) was used as a nuclear counterstain at 1 µg/ml. DAPT (γ-secretase inhibitor, D5942), Thio avine S (T1892), Anti-Digoxigenin-AP (Fab fragments, 11093274910), and Phorbol 12-myristate 13-acetate (PMA, p8139) were ordered from Sigma-Aldrich.