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Taqman probes

Manufactured by Metabion
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TaqMan probes are oligonucleotide sequences used in real-time PCR (polymerase chain reaction) assays. They are designed to specifically hybridize to a target DNA sequence and their fluorescent signal is detected during the PCR amplification process, allowing for quantitative measurement of the target DNA.

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Lab products found in correlation

Luminaris color probe qpcr master mix, by Thermo Fisher Scientific (2 mentions) Quick dna universal kit, by Zymo Research (2 mentions) 7500 fast system, by Thermo Fisher Scientific (1 mentions) Abi prism 3100 genetic analyzer, by Thermo Fisher Scientific (1 mentions) Cfx96 real time detection system, by Bio-Rad (1 mentions)

3 protocols using taqman probes

1

Characterization of β-Lactamase-Producing Bacteria

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For this study, 12 Enterobacterales and 3 Pseudomonas aeruginosa strains (15 in total) belonging to the bacterial collection of the Department of Application Development Microbiology & Diagnostics, Bruker Daltonik GmbH in Bremen (Germany), were used. All strains were previously characterized as β-lactamase-producing bacteria (BLPB) using validated routine PCR methods (TaqMan-probe realtime-PCR using the KAPA™ PROBE FAST qPCR MasterMix Universal (peqlab), Primer and TaqMan-Probes (both metabion) using an ABI 7500 Fast System) and showed the presence of different classes and types of β-lactamases (BLs) coding genes–including Ambler class A & D serine carbapenemases as well as class B β-metallo-carbapenemases (Table 1).
Złoch M., Pomastowski P., Peer M., Sparbier K., Kostrzewa M, & Buszewski B. (2021). Study on carbapenemase-producing bacteria by matrix-assisted laser desorption/ionization approach. PLoS ONE, 16(3), e0247369.
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2

Genotyping CYP2C9 and CYP2C8 Variants

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Genomic DNA was isolated from liver samples by Quick-DNA™ Universal Kit (Zymo Research, Irvine, CA). Hydrolysis SNP analysis for CYP2C9*2, CYP2C9*3 and CYP2C8*3 was performed by polymerase chain reaction (PCR) with TaqMan probes (Metabion, Planegg/Steinkirchen, Germany) as previously reported29 (link). Real-time PCR was carried out with 30 ng of genomic DNA by using Luminaris Color Probe qPCR Master Mix (Thermo Fisher Scientific, Waltham, MA).
Fekete F., Mangó K., Déri M., Incze E., Minus A, & Monostory K. (2021). Impact of genetic and non-genetic factors on hepatic CYP2C9 expression and activity in Hungarian subjects. Scientific Reports, 11, 17081.
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3

Genotyping of CYP2C19 Variants

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Hydrolysis SNP analysis for CYP2C19*2, CYP2C19*3, CYP2C19*4 and CYP2C19*17 was performed by polymerase chain reaction (PCR) with TaqMan probes (Metabion, Planegg/Steinkirchen, Germany) using a CFX96 real-time detection system (Bio-Rad Laboratories). Primers and probes (Table 2) were designed based on the reference SNP sequences in the National Center for Biotechnology Information reference assembly.
Genomic DNA was isolated from liver samples by Quick-DNA™ Universal Kit (Zymo Research, Irvine, CA). PCR was carried out with 30 ng of genomic DNA by using Luminaris Color Probe qPCR Master Mix (Thermo Fisher Scientific, Waltham, MA). To confirm the results of CYP2C19 genotyping, a sequence analysis was performed. The PCR products were sequenced directly in an ABI PRISM 3100 Genetic Analyzer (Applied Biosystems, Foster City, CA) by the Sequencing Service of Biomi Ltd. (Gödöllő, Hungary).
Kiss Á.F., Vaskó D., Déri M.T., Tóth K, & Monostory K. (2018). Combination of CYP2C19 genotype with non-genetic factors evoking phenoconversion improves phenotype prediction. Pharmacological reports : PR, 70(3).
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