Log In Sign up
The largest database of trusted experimental protocols

Mouse plus and rabbit minus probes

Manufactured by Merck Group
Visit Supplier

The Mouse PLUS and Rabbit MINUS probes are laboratory reagents designed for use in various scientific applications. The Mouse PLUS probe is a molecular tool used for the detection and analysis of target molecules, while the Rabbit MINUS probe serves a similar purpose for a different set of targets. These probes are commonly utilized in techniques such as immunoassays, gene expression studies, and diagnostic procedures. Their core function is to facilitate the identification and quantification of specific analytes in a sample, but the detailed applications may vary depending on the specific research or diagnostic needs.

Automatically generated - may contain errors

Lab products found in correlation

In situ pla detection kit, by Merck Group (2 mentions) Lsm 710 microscope, by Zeiss (1 mentions)

Close spelling variants of this product

Anti-Mouse PLUS and anti-Rabbit MINUS PLA Probes (3 citations) Duolink anti-Mouse MINUS and anti-Rabbit PLUS In Situ PLA probes (2 citations)

2 protocols using mouse plus and rabbit minus probes

1

In situ Proximity Ligation Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed 3 times in PB and incubated in mouse PLUS and rabbit MINUS probes (Sigma Aldrich) according to the manufacturer’s protocol. Cells were washed in PB before detection using the in situ PLA detection kit (Sigma-Aldrich) as previously described (van Loosdregt et al., 2011 (link)). Samples were analyzed with a 63x objective on a Zeiss LSM 710 microscope (Carl Zeiss).
Fleskens V., Minutti C.M., Wu X., Wei P., Pals C.E., McCrae J., Hemmers S., Groenewold V., Vos H.J., Rudensky A., Pan F., Li H., Zaiss D.M, & Coffer P.J. (2019). Nemo-like Kinase Drives Foxp3 Stability and Is Critical for Maintenance of Immune Tolerance by Regulatory T Cells. Cell Reports, 26(13), 3600-3612.e6.
+ Open protocol
+ Expand
2

In Situ Proximity Ligation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed 3 times in PB and incubated in mouse PLUS and rabbit MINUS probes (Sigma Aldrich) according to the manufacturer's protocol. Cells were washed in PB before detection using the in situ PLA detection kit (Sigma-Aldrich) as previously described 37 . Samples were analyzed with a 63x objective on a Leica SP8X.
Mocholí E., Russo L., Gopal K., Ramstead A.G., Hochrein S.M., Vos H.R., Geeven G., Adegoke A., Hoekstra A.T., Es R.v., Pittol J.M.R., Vastert S.J., Rutter J., Radstake T.R.D.J., Loosdregt J.v., Berkers C.R., Mokrý M., Anderson C.C., O’Connell R.M., Vaeth M., Ussher J.R., Burgering B., & Coffer P.J. (2022). Pyruvate metabolism controls epigenome remodeling during CD4+ T cell activation.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!