Total RNA was isolated from tissue blocks of corpus callosum/hippocampus using the Trizol Technique (Life Technologies, Invitrogen) and RNeasy Mini Kit 250 (Qiagen). Reverse transcription was performed using SuperScript II Reverse Transcriptase and dNTP set (Invitrogen), Random primers and RNasin (Promega) and followed by qPCR using the TaqMan Gene Expression Assays: PCR Master Mix, predesigned Taqman primers for MBP (Inventoried) and endogenous control (GAPDH), Thermo-Fast 96, and ABgene plates (Applied Biosystems, Thermofisher). Expression of genes was analyzed with the 7300 Systems SDS Software (Applied Biosystem) and MBP expression was normalized to reference gene GAPDH, whose expression was stable in different groups and in two independent experiments.
7300 systems sds software
Manufactured by Thermo Fisher Scientific
The 7300 Systems SDS Software is a software package designed for use with Thermo Fisher Scientific's 7300 Real-Time PCR System. The software provides an interface for instrument control, data analysis, and reporting for the 7300 Real-Time PCR System.
Automatically generated - may contain errors
2 protocols using 7300 systems sds software
1
Quantifying Myelination Markers in Tissues
2
Quantification of Oligodendrocyte Markers
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Total RNA was extracted from blocks of corpus callosum/hippocampus of 1‐week‐treated animals or controls, using the Trizol Technique (Life technologies, Invitrogen) and RNeasy mini‐kits 250 (Quiagen). Reverse transcription was performed using Superscript II reverse transcriptase and dNTP set (Invitrogen), Random primers and RNasin (Promega) and followed by qPCR using the TaqMan Gene Expression Assays: PCR master Mix, predesigned Taqman primers for Myt1, Olig2 and Sox17, and endogenous control (GAPDH), Thermo‐Fast 96 and ABgene plates (Applied Biosystems, Thermofisher). Expression of genes was analyzed with the 7300 Systems SDS Software (Applied Biosystem) and the expression of Myt1, Olig2, and Sox17 normalized to reference gene GAPDH whose expression was stable in different groups.
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