D erythro sphingosine c17 base

Manufactured by Avanti Polar Lipids

Sourced in United States

D-erythro-sphingosine (C17 base) is a naturally occurring sphingolipid that can be used in various laboratory applications. It is a key structural component of cell membranes and plays a role in cellular signaling processes. This product is suitable for research purposes, but its specific applications and intended uses should not be extrapolated beyond the provided factual description.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Vinblastine sulfate, by Merck Group (1 mentions) Bovine calf serum (bcs), by Thermo Fisher Scientific (1 mentions) Gentamycin, by Merck Group (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin solution p s, by Thermo Fisher Scientific (1 mentions) Cxcl10, by Fujifilm (1 mentions) Sodium hydroxide (naoh), by Merck Group (1 mentions) Calcium chloride, by Merck Group (1 mentions) Acetonitrile, by Avantor (1 mentions) Protease inhibitor cocktail, by Merck Group (1 mentions) Sucrose, by Merck Group (1 mentions) Potassium chloride (kcl), by Merck Group (1 mentions) Sodium chloride (nacl), by Merck Group (1 mentions) D glucose, by Merck Group (1 mentions) 1 palmitoyl 2 hydroxy sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) 1 stearoyl 2 hydroxy sn glycero 3 phosphocholine, by Avanti Polar Lipids (1 mentions) Propidium iodide, by Thermo Fisher Scientific (1 mentions) Annexin 5, by Thermo Fisher Scientific (1 mentions) Thiazolyl blue tetrazolium bromide mtt, by Avantor (1 mentions)

Close spelling variants of this product

C17-sphingosine (27 citations) D-erythro-sphingosine (6 citations)

2 protocols using d erythro sphingosine c17 base

Lipid Extraction and Purification Protocol

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Metrizamide was purchased from Amresco (Solon, OH). Vinblastine sulfate, sucrose, gentamycin, Trizma-hydrochloric acid, potassium chloride, sodium chloride, d-glucose, calcium chloride, protease inhibitor cocktail (for mammalian cell and tissue extracts, in DMSO solution), sodium hydroxide, and magnesium chloride were from Sigma Aldrich (St. Louis, MO). Glycyl-L-phenylnaphthylamine was from Bachem (Torrance, CA). Methanol was from Fisher Scientific (Fairlawn, NJ). Dichloromethane was from Mallinkrodt (Phillipsburg, NJ). Ultra LC/MS-grade Methanol, acetonitrile, and water were from JT Baker (Center Valley, PA). Formic acid was from EMD (Darmstadt, Germany). Penicillin-streptomycin solution (PS) was from Life Technologies (Grand Island, NY). Anti-TNP IgE antibody was from BD Biosciences (San Jose, CA). CXCL10 was from Shenandoah Biotechnology (Warwick, PA). Dubelco’s Modified Eagle Medium (DMEM) high glucose cell medium, bovine calf serum, and fetal bovine serum were from Thermo Scientific (Waltham, MA). Standards including: 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphocholine, 1-stearoyl-2-hydroxy-sn-glycero-3-phosphocholine, 1-hexadecanoyl-sn-glycero-3-phosphoethanolamine, and D-erythro-sphingosine (C17 base) were purchased from AvantiPolarLipids (Alabaster, AL).

Satori C.P., Ramezani M., Koopmeiners J.S., Meyer A.F., Rodriguez-Navarro J.A., Kuhns M.M., Taylor T.H., Haynes C.L., Dalluge J.J, & Arriaga E.A. (2016). Checkpoints for Preliminary Identification of Small Molecules found Enriched in Autophagosomes and Activated Mast Cell Secretions Analyzed by Comparative UPLC/MSe. Analytical methods : advancing methods and applications, 9(1), 46-54.

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Evaluating Apoptosis Induction in Cells

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Lipofectamine^® RNAiMAX, Annexin‐V, and propidium iodide were purchased from Life Technologies (Grand Island, NY, USA). X‐tremeGENE 9 DNA Transfection Reagent was purchased from Roche Diagnostics (Indianapolis, IN, USA). iTAQ and SYBR^® Green master mix master mix was purchased from Bio‐Rad (Hercules, CA, USA). CHK1/2 inhibitor AZD7762 was purchased from sSelleckchem (Houston, TX, USA). Doxorubicin hydrochloride and cycloheximide were purchased from Sigma Aldrich (St. Louis, MO, USA). D‐Erythro‐sphingosine (C17 base) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Thiazolyl blue tetrazolium bromide (MTT) was purchased from Amresco (Solon, OH, USA). SKi‐II (4‐[4‐(4‐chloro‐phenyl)‐thiazol‐2‐ylamino]‐phenol) was purchased from Cayman Chemical (Ann Arbor, MI, USA).

Carroll B.L., Bonica J., Shamseddine A.A., Hannun Y.A, & Obeid L.M. (2017). A role for caspase‐2 in sphingosine kinase 1 proteolysis in response to doxorubicin in breast cancer cells – implications for the CHK1‐suppressed pathway. FEBS Open Bio, 8(1), 27-40.

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