Gastric cancer cell lines were purchased from ATCC (KATOIII, NCI-N87, SNU-16), KCLB (SNU-668, SNU-601, SNU-638), JCRB (MKN-45, NUGC-4) and ECACC (HGC-27). Microsatellite Instability (MSI) status was previously assessed for all cell lines and found negative for all but one cell line (SNU-638). Identity of each cell line was determined through independent karyotyping. Cells were checked for mycoplasma contamination. Cells were cultured in their recommended media conditions at 37°C. Afterward, the cells were processed into suspensions with standard procedures. Briefly, this process involved trypsinizing the cells, followed by inactivation by fetal bovine serum (FBS). We performed washes by centrifugation at 400 g in 1× phosphate-buffered saline with 0.04% bovine serum albumin. To remove cellular debris and cellular aggregates, we filtered cells through a Flowmi cell strainer (Wayne, NJ, USA) before proceeding to single-cell DNA and RNA sequencing.
Snu638
Manufactured by American Type Culture Collection
Sourced in United States
The SNU638 is a laboratory equipment product designed for cell culture applications. It provides a controlled environment for the growth and maintenance of various types of cells. The core function of the SNU638 is to maintain a stable temperature, humidity, and atmospheric conditions suitable for cell culture.
Automatically generated - may contain errors
Lab products found in correlation
Hct116, by American Type Culture Collection (7 mentions)
Sk hep1, by American Type Culture Collection (6 mentions)
Mda mb 231, by American Type Culture Collection (6 mentions)
Nci n87, by American Type Culture Collection (5 mentions)
Mkn45, by American Type Culture Collection (3 mentions)
Kato 3, by American Type Culture Collection (3 mentions)
Streptomycin, by Thermo Fisher Scientific (3 mentions)
Hek293, by American Type Culture Collection (3 mentions)
Snu 1, by American Type Culture Collection (3 mentions)
Snu 16, by American Type Culture Collection (2 mentions)
Fetal bovine serum (fbs), by System Biosciences (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Mrc 5, by American Type Culture Collection (2 mentions)
Ges 1, by American Type Culture Collection (2 mentions)
Sw620, by American Type Culture Collection (2 mentions)
Hct116, by Thermo Fisher Scientific (2 mentions)
Snu 601, by American Type Culture Collection (1 mentions)
Nugc4, by American Type Culture Collection (1 mentions)
Hgc 27, by American Type Culture Collection (1 mentions)
Hep3b, by American Type Culture Collection (1 mentions)
13 protocols using snu638
1
Characterization of Gastric Cancer Cell Lines
2
Characterization of Cancer Cell Lines
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The following human cancer cell lines were obtained from the American Type Culture Collection (ATCC) or the Korean Cell Line Bank (KCLB): The liver cancer cells, HepG2 (ATCC HB-8065), Hep3B (ATCC HB-8064), PLC/PRF/5 (ATCC CRL-8024), Huh7 (KCLB 60104) and SK-HEP1 (ATCC HTB-52); the gastric cancer cells, SNU638 (KCLB 00638); the lung cancer cells, A549 (ATCC CRM-CCL-185); the colorectal cancer cells, HT-29 (ATCC HTB-38); the prostate cancer cells, LNCaP/LN3 (KCLB 80018); the cervical cancer cells, HeLa (ATCC CRM-CCL-2); and the breast cancer cells, SK-BR-3 (ATCC HTB-30). The cells were cultured in Dulbecco's modified Eagle's medium (DMEM) or RPMI-1640 (Invitrogen; Thermo Fisher Scientific, Inc.) supplemented with 10% inactivated fetal bovine serum (FBS; Sigma-Aldrich). Conditioned media containing exosomes were prepared from 70 to 80% confluent HepG2 cells, which were cultured in complete DMEM for 48 h with 10% exosome-free FBS (System Biosciences, LLC). The cell lines (HepG2, HT-29 and SK-HEP1) were authenticated using short tandem repeat (STR) analysis by the Korean Cell Line Bank (KCLB), and the STR profile results are presented in Data S1.
3
Gastric Cancer Cell Culture Protocol
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MKN74, MKN28, NCI-N87, SNU638, and AGS, were bought from the ATCC and were maintained in 1640 contained with 10% fetal bovine serum (FBS, Life Technologies, Grand Island, NY), 100 mg/ml penicillin G and 50 mg/ml streptomycin (Life Technologies, Grand Island, NY) at 37 °C in a humidified atmosphere containing 5% CO2. MKN28 and MKN74 cells were treated with corresponding GANT61 (GAN, ENZO Lifesciences).
4
Cell Culture Conditions for Cancer Research
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HCT116 (human colorectal cancer cells), A549 (human lung cancer cells), SK-HEP-1 (human liver cancer cells), K562 (human erythroleukemic cells), SNU638 (human gastric cancer cells), MDA-MB-231 (human breast cancer cells), HEK293 (human embryonic kidney cells), and MRC5 (human normal lung epithelial cells) were purchased from the American Type Culture Collection (Manassas, VA, USA). The cells were cultured in the medium (RPMI 1640 for HCT116, A549, K562, and SNU638 cells; DMEM for SK-HEP-1, MDA-MB-231, and HEK293 cells; MEM for MRC-5 cells) supplemented with 10% FBS and antibiotics-antimycotics (PSA; 100 units/mL penicillin G sodium, 100 μg/mL streptomycin, and 250 ng/mL amphothericin B) in a 37°C humidified incubator with 5% CO2.
5
Culturing Human Cancer Cell Lines
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Human colorectal cancer cells (HCT116), gastric cancer cells (SNU638), breast cancer cells (MDA-MB-231), and liver cancer cells (SK-HEP-1) were purchased from American Type Culture Collection (Manassas, VA, USA). HCT116 and SNU638 cells were maintained in RPMI1640 medium, while MDA-MB-231 and SK-HEP-1 cells were cultured in DMEM medium that was supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 units/mL penicillin, 100 μg/mL streptomycin, and 250 ng/mL amphotericin B, respectively. Cells were maintained at 37 °C in a humidified atmosphere with 5% CO2.
6
Cell Culture Practices for Cancer Research
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All the cancer cell lines (SNU-638, SK-HEP-1, A549, HCT116, and MDA-MB-231 cancer cells) were obtained from the American Type Culture Collection (Manassas, VA, USA). SNU-638, A549, and HCT116 cells were cultured in Roswell Park Memorial Institute 1640 medium, and SK-HEP-1 and MDA-MB-231 cells were cultured in Dulbecco’s Modified Eagle’s medium. All media were supplemented with a penicillin–streptomycin mixture (10,000 units/mL sodium penicillin G and 10,000 μg/mL streptomycin) and 10% fetal bovine serum. They were incubated in a humidified incubator that contained 5% CO2 at 37 °C.
7
Gastric Cancer Cell Line Cultivation
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GC cell lines (AGS, NCI-N87, MKN1, MKN45, and SNU638) were purchased from the American Type Culture Collection or Korean Cell line Bank. The normal gastric epithelial cell line, HFE145 was generated by Drs. Duane T Smoot and Hassan Ashktorab [26 (link)]. Cells were grown in Dulbecco's modified Eagle's essential medium (DMEM) or RPMI 1640 medium supplemented with 10% fetal bovine serum. Cells were incubated at 37°C in a humidified incubator with 5% CO2. VP (#1711461) was purchased from U.S. Pharmacopeial Convention (Rockville, MD, USA).
8
Gastric Cancer Cell Line Culture
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Human GC cell lines SNU-1, SNU-638, BGC-823, SGC-7901, KATOIII and normal gastric epithelial cell line (GES-1) were purchased from American Type Culture Collection (ATCC). MKN-28, HGC-27 and MGC-803 cell lines were donated by Shanghai Cancer Institute and Renji Hospital Affiliated to Shanghai Jiaotong University School of Medicine. The cell lines were cultured in RPMI1640 medium (HyClone) containing 10% fetal bovine serum (FBS, GIBCO), 2 mM l -glutamine, 100U ml−1 penicillin and 100 μg ml−1 streptomycin. The cells were incubated in an atmosphere of 5% CO2 at 37 °C.
9
Gastric and Colon Cancer Cell Lines
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The gastric cancer cell lines (AGS, MKN28, MKN45, SNU1, SNU16, SNU638, SNU719, MGC803, and NCI-N87) and immortalized normal human gastric epithelial cell line (GES1) were purchased from the American Type Culture Collection (ATCC; Manassas, VA, USA) and the Cancer Research Institute of Beijing University, China. The cell lines were cultured in RPMI-1640 medium (Gibco; Life Technologies, Carlsbad, CA, USA) with 10% fetal bovine serum (FBS) in 5% CO2 at 37 °C.
The colon cancer cell line HCT116 p53-wild type cells were purchased from ATCC and HCT116 p53-knockout cells were kindly provided by Professor Bert Vogelstein from the Ludwig Center and the Howard Hughes Medical Institute at the Johns Hopkins University School of Medicine. HCT116 p53-wild type cells were cultured in McCoy's 5A medium (Sigma-Aldrich, St Louis, MO, USA) with 10% FBS and HCT116 p53-knockout cells were cultured in Dulbecco's modified Eagle's medium (Gibco; Life Technologies) with 10% FBS in 5% CO2 at 37 °C.
The colon cancer cell line HCT116 p53-wild type cells were purchased from ATCC and HCT116 p53-knockout cells were kindly provided by Professor Bert Vogelstein from the Ludwig Center and the Howard Hughes Medical Institute at the Johns Hopkins University School of Medicine. HCT116 p53-wild type cells were cultured in McCoy's 5A medium (Sigma-Aldrich, St Louis, MO, USA) with 10% FBS and HCT116 p53-knockout cells were cultured in Dulbecco's modified Eagle's medium (Gibco; Life Technologies) with 10% FBS in 5% CO2 at 37 °C.
10
Cell Culture Conditions for Various Human Cell Lines
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Human embryonic kidney cells (HEK293), human normal lung fibroblast cell (MRC-5), human colorectal cancer cells (HCT116), human breast cancer cells (MDA-MB-231), human lung cancer cells (A549), human liver cancer cells (SK-HEP-1), and human gastric cancer cells (SNU-638) were purchased from the American Type Culture Collection (Manassas, VA, USA). HEK293, MRC5, MDA-MB-231 and SK-HEP-1 were maintained in DMEM, while HCT116, A549 and SNU-638 were cultured in RPMI-1640 media supplemented with 10% FBS and antibiotics-antimycotics (PSF: 100 units/mL sodium penicillin G, 100 μg/mL streptomycin, and 250 ng/mL amphotericin B) (Thermo-Scientific, MA, USA) in a humidified incubator containing 5% CO2 at 37°C.
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