Pifithrin α

Manufactured by Adipogen

Pifithrin-α is a small-molecule inhibitor of p53-mediated transcriptional activation and apoptosis. It functions by inhibiting the transcriptional activity of p53, a key regulator of cellular processes such as cell cycle arrest, apoptosis, and DNA repair.

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3 protocols using pifithrin α

Evaluating p53 Ubiquitination Pathways

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Picrotoxin and poly-D-lysine were from Santa Cruz Biotechnology. Pifithrin-α was from Adipogen Corporation. Nutlin-3 was from Cayman Chemical. Dimethyl sulfoxide (DMSO) was from Fisher Scientific. The antibodies used in this study were purchased from Santa Cruz Biotechnology (andi-p53 and IgG controls), GenScript Corporation (anti-Gapdh), and Cell Signaling (anti-Ubiquitin and anti-p53). HRP-conjugated secondary antibodies were from Santa Cruz Biotechnology and Sigma.

Jewett K.A., Lee K.Y., Eagleman D.E., Soriano S, & Tsai N.P. (2018). Dysregulation and restoration of homeostatic network plasticity in fragile X syndrome mice. Neuropharmacology, 138, 182-192.

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Comprehensive Protein Regulation Analysis

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Cycloheximide was from Santa Cruz Biotechnology. Actinomycin-D was from Sigma. MG132 was from Selleck Chemical. Puromycin was from MP Biomedicals. Kainic acid and Thapsigargin were from Alomone Lab. Pifithrin-α was from Adipogen Corporation. Salubrinal was from Enzo Life Sciences. Dimethyl sulfoxide (DMSO) was from Fisher Scientific. DMSO was used as a vehicle in this study. The antibodies used in this study were purchased from Santa Cruz Biotechnology (anti-Mdm2, anti-Lamin A/C, anti-HSP-90), GenScript Corporation (anti-Gapdh), Millipore (anti-Puromycin), and Cell Signaling (anti-ubiquitin, anti-p-Mdm2, anti-p53, anti-BiP, anti-PDI, anti-PERK, anti-ATF6, anti-XBP1s, anti-eIF2α and anti-p-eIF2α). HRP-conjugated secondary antibodies were from Santa Cruz Biotechnology and Cell Signaling. The lentiviral control non-target shRNA and p53 shRNA were from Santa Cruz Biotechnology.

Liu D.C., Eagleman D.E, & Tsai N.P. (2019). Novel roles of ER stress in repressing neural activity and seizures through Mdm2- and p53-dependent protein translation. PLoS Genetics, 15(9), e1008364.

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Picrotoxin and p53 Inhibitor Study

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Picrotoxin (PTX) was from Santa Cruz Biotechnology. Dimethyl sulfoxide (DMSO) was from Fisher Scientific. Pifithrin-α was from Adipogen Corporation. Nutlin-3 was from Cayman Chemical. PTX was dissolved in methanol while Pifithrin-α and Nutlin-3 were dissolved in DMSO. DMSO and methanol were therefore used as vehicles in this study. The antibodies used in this study were purchased from Santa Cruz Biotechnology (anti-p53 and anti-Mdm2), Cell Signaling (anti-Nedd4-2 and anti-phospho-Mdm2) GenScript Corporation (anti-Gapdh), StressMarq (anti-VGLUT1) and Abgent (anti-GAD65).

Jewett K.A., Christian C.A., Bacos J.T., Lee K.Y., Zhu J, & Tsai N.P. (2016). Feedback modulation of neural network synchrony and seizure susceptibility by Mdm2-p53-Nedd4-2 signaling. Molecular Brain, 9, 32.

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