Ti saphirelaser

Manufactured by Spectra-Physics

The Ti-SaphireLaser is a versatile laser system that produces high-intensity, tunable light in the near-infrared region of the electromagnetic spectrum. It utilizes a titanium-doped sapphire crystal as the active medium, providing a wide range of wavelength options from approximately 670 to 1080 nanometers.

Automatically generated - may contain errors

Lab products found in correlation

Lsm 700, by Zeiss (2 mentions) Model 3980, by Spectra-Physics (1 mentions)

2 protocols using ti saphirelaser

Fluorescence Lifetime Measurements by Streak Camera

Check if the same lab product or an alternative is used in the 5 most similar protocols

The fluorescence lifetimes
were obtained by streak-camera measurements. A frequency doubled Ti-Saphire
Laser (Tsunami, Newport Spectra-Physics GmbH) was used as the light
source to generate the 390 nm excitation pulses. The pulse-to-pulse
repetition rate of the laser was reduced to 4 kHz by a pulse selector
(Model 3980, Newport Spectra-Physics GmbH). The emission was recorded
using a CHROMEX 250IS spectrograph and a Hamamatsu HPD-TA streak-camera.
The power of the pump-pulse used was in the range of 0.2 mW, and the
OD of the sample at the excitation wavelength was in the range of
0.05 to 0.1.

Nair S.S., Bysewski O.A., Klosterhalfen N., Sittig M., Winter A., Schubert U.S, & Dietzek-Ivanšić B. (2024). Intramolecular Energy Transfer Competing with Light-Driven Intermolecular Proton Transfer in an Iron(II)-NHC Complex? A Query into the Role of Photobasic Ligands and MLCT States. ACS Omega, 9(11), 13427-13439.

+ Open protocol

+ Expand

Imaging Mitochondrial Dynamics in Neuronal Processes

Check if the same lab product or an alternative is used in the 5 most similar protocols

MitoDendra+ mitochondria in neuronal processes (excluding somatic mitochondria) in organotypic slices were live-imaged using the 20× and 60× water objectives on a Zeiss LSM700 upright two-photon microscope equipped with a MaiTai Ti:Saphire Laser (Spectra-Physics). The slices were transferred to a recording chamber, perfused with aCSF (2 mM CaCl₂, 2.5 mM KCl, 1 mM MgCl₂, 10 mM D-glucose, 126 mM NaCl, 24 mM NaHCO₃, 1 mM NaH₂PO₄) bubbled with carbogen gas and heated between 35 and 38°C at a constant perfusion (∼2 ml/min). The excitation wavelength was set at 900 nm, and the rate of image acquisition was one frame/5 s for 500 s (100 frames/movie).

Kontou G., Antonoudiou P., Podpolny M., Szulc B.R., Arancibia-Carcamo I.L., Higgs N.F., Lopez-Domenech G., Salinas P.C., Mann E.O, & Kittler J.T. (2021). Miro1-dependent mitochondrial dynamics in parvalbumin interneurons. eLife, 10, e65215.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!