Liver tissues were fixed in 10% buffered formalin, embedded in paraffin and stained with hematoxylin and eosin. To visualize collagen fibers, liver sections were stained with saturated picric acid containing 0.5% Sirius Red (Sigma). The sections were washed with distilled water, dehydrated, mounted and analyzed by light microscopy. For immunostaining of BiP, p-PERK and F4/80, paraffin-embedded liver sections were incubated with corresponding primary antibodies (1:200, 1:50 and 1:100, respectively), followed by incubation with biotin-conjugated secondary antibodies (1:200) and streptavidin-HRP (1:300). The HRP activity was visualized by diaminobenzidine (DAB) staining. Hematoxylin counterstaining was applied to visualize nuclei. For α-SMA staining, liver sections were incubated with anti-α-SMA antibody (1:100), followed by incubation with Alexa Flour 594-conjugated secondary antibody (1:250) and counterstaining with DAPI (Invitrogen). TUNEL assay was performed using In Situ Cell Death Detection Kit-TMR-Red (Roche). Samples were analyzed under an epifluorescence-equipped light microscope (Olympus).
Epifluorescence equipped light microscope
Manufactured by Olympus
The Epifluorescence-equipped light microscope is a specialized laboratory instrument designed for the observation and analysis of fluorescently labeled samples. It utilizes a combination of illumination and detection techniques to visualize the fluorescent signals emitted by the sample, providing a powerful tool for various applications in life sciences and materials research.
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Lab products found in correlation
4 protocols using epifluorescence equipped light microscope
1
Histological Analysis of Liver Fibrosis
2
Immunofluorescence Analysis of c/EBPβ
Check if the same lab product or an alternative is used in the 5 most similar protocols
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For c/EBPβ staining, HepG2 cells were fixed with methanol and incubated with anti-c/EBPβ antibody (1:100), followed by incubation with Alexa Fluor 594-conjugated secondary antibody (1:500) and counterstaining with DAPI (Invitrogen). Samples were analyzed under an epifluorescence-equipped light microscope (Olympus).
3
Histological Analysis of Liver Fibrosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liver tissues were fixed in 10% buffered formalin, embedded in paraffin and stained with hematoxylin and eosin. To visualize collagen fibers, liver sections were stained with saturated picric acid containing 0.5% Sirius Red (Sigma). The sections were washed with distilled water, dehydrated, mounted and analyzed by light microscopy. For immunostaining of BiP, p-PERK and F4/80, paraffin-embedded liver sections were incubated with corresponding primary antibodies (1:200, 1:50 and 1:100, respectively), followed by incubation with biotin-conjugated secondary antibodies (1:200) and streptavidin-HRP (1:300). The HRP activity was visualized by diaminobenzidine (DAB) staining. Hematoxylin counterstaining was applied to visualize nuclei. For α-SMA staining, liver sections were incubated with anti-α-SMA antibody (1:100), followed by incubation with Alexa Flour 594-conjugated secondary antibody (1:250) and counterstaining with DAPI (Invitrogen). TUNEL assay was performed using In Situ Cell Death Detection Kit-TMR-Red (Roche). Samples were analyzed under an epifluorescence-equipped light microscope (Olympus).
4
Immunofluorescence Analysis of c/EBPβ
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