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Rabbit igg 12 370

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Rabbit IgG (12-370) is a laboratory reagent that contains rabbit-derived immunoglobulin G (IgG). IgG is the most abundant antibody isotype found in the blood and plays a crucial role in the adaptive immune response. This product is typically used in various immunological assays and techniques that require the use of an IgG antibody.

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Lab products found in correlation

Chip kit, by Merck Group (2 mentions) Gata3 sc 22206, by Santa Cruz Biotechnology (2 mentions) Hiseq 2000 sequencer, by Illumina (2 mentions) Ab23980, by Abcam (2 mentions) Sc 9737, by Santa Cruz Biotechnology (2 mentions) C ebpα sc 61, by Santa Cruz Biotechnology (2 mentions) Protein a g agarose beads, by Merck Group (1 mentions) Ls c30343, by LSBio (1 mentions)

Spelling variants of this product

IgG rabbit (4 citations)

5 protocols using rabbit igg 12 370

1

Chromatin Immunoprecipitation of Th Cell Differentiation

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Naive CD4+CD25−CD62LhiCD44lo T cells were isolated and activated under Th0 or Th2 differentiation conditions. Four days later cells were restimulated with anti-CD3 (2μg/ml) for 4 hours and fixed with 1% formaldehyde for 10 minutes at 37°C. Chromatin immunoprecipitation was performed using the Millipore ChIP kit. Briefly, chromatin was seared (500bp-100kb) and immunoprecipitated using the Abs to the following: AcH3 (06-599), H3k4me3 (07-473), H3k27me3 (074-449), Rabbit IgG (12-370) (Millipore, 2.5μg/sample), Stat6 (sc-981), IRF4 (sc-6059), JunB (sc-73) and Gata3 (sc-22206) (Santa Cruz Biotechnology, 5μg/sample). Following reversal of cross-links, the presence of selected DNA sequences was assessed by qRT-PCR using the following primer sequences: Grail promoter (primer1 - F-TTTTAAGAGGCGGATCCCGCA; R-GCTGGGACTAGGTGCGAACCAG); primer 2 - F-AAATCGAGGCGCAGAGAAGTAA; R-CTAAAATGAGGCCAGCCCACCT), IL-4 promoter (Histone and Stat6 ChIP respectively)35 (link)–36 (link), HS2 locus37 , CNS-3 (IL-10 locus)38 (link), CNS+6.45(IL-10 locus)39 (link).
Sahoo A., Alekseev A., Obertas L, & Nurieva R. (2014). Grail controls Th2 cell development by targeting STAT6 for degradation. Nature communications, 5, 4732.
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2

Immunoprecipitation and Western Blot Analysis of Jmjd6 in Embryos

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Embryos were homogenized in RIPA buffer [10 mM Tris-Cl (pH 8.0), 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.1% SDS, 140 mM NaCl] in the presence of complete protease inhibitors (Roche) and 1mM phenylmethylsulphonylfluoride. Yolk was removed by centrifugation for 10 min at 4°C. The extract was incubated with anti-Jmjd6 antibody (LS-C30343, LSbio) for overnight at 4°C and subsequently incubated with protein A/G agarose beads (Sigma‐Aldrich) for 4 hr at 4°C. The beads were washed extensively and mixed with SDS sample buffer. For negative control, rabbit IgG (12–370, Millipore) was used. Western blotting was performed with anti-Jmjd6 (LS-C30343, LSbio, 1:1000), anti-α/β tubulin (2148, Cell Signaling, 1:1000), anti-U2AF65 (ab151582, Abcam, 1:1000), anti-β-catenin (610154, BD Transduction Laboratories, 1:1000), anti-phospho-β-catenin (Ser33/37/Thr41) (9561S, Cell Signaling Technology, 1:1000), and anti-GSK3β (SC-81462, SC-71186, Santa Cruz Biotechnology, 1:1000) antibodies.
Shin J.Y., Son J., Kim W.S., Gwak J, & Ju B.G. (2019). Jmjd6a regulates GSK3β RNA splicing in Xenopus laevis eye development. PLoS ONE, 14(7), e0219800.
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3

Chromatin Immunoprecipitation of Th Cell Differentiation

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Naive CD4+CD25−CD62LhiCD44lo T cells were isolated and activated under Th0 or Th2 differentiation conditions. Four days later cells were restimulated with anti-CD3 (2μg/ml) for 4 hours and fixed with 1% formaldehyde for 10 minutes at 37°C. Chromatin immunoprecipitation was performed using the Millipore ChIP kit. Briefly, chromatin was seared (500bp-100kb) and immunoprecipitated using the Abs to the following: AcH3 (06-599), H3k4me3 (07-473), H3k27me3 (074-449), Rabbit IgG (12-370) (Millipore, 2.5μg/sample), Stat6 (sc-981), IRF4 (sc-6059), JunB (sc-73) and Gata3 (sc-22206) (Santa Cruz Biotechnology, 5μg/sample). Following reversal of cross-links, the presence of selected DNA sequences was assessed by qRT-PCR using the following primer sequences: Grail promoter (primer1 - F-TTTTAAGAGGCGGATCCCGCA; R-GCTGGGACTAGGTGCGAACCAG); primer 2 - F-AAATCGAGGCGCAGAGAAGTAA; R-CTAAAATGAGGCCAGCCCACCT), IL-4 promoter (Histone and Stat6 ChIP respectively)35 (link)–36 (link), HS2 locus37 , CNS-3 (IL-10 locus)38 (link), CNS+6.45(IL-10 locus)39 (link).
Sahoo A., Alekseev A., Obertas L, & Nurieva R. (2014). Grail controls Th2 cell development by targeting STAT6 for degradation. Nature communications, 5, 4732.
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4

ChIP-seq for Transcription Factors

Cited 1 time
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ChIP assays were performed as described previously (Ptasinska et al., 2012 (link)). Nuclei were essentially prepared as described previously (Lefevre et al., 2003 (link)). The following antibodies were used: C/EBPα, SC-61 (Santa Cruz Biotechnology); ETO (C terminus specific), SC-9737 (Santa Cruz); HDAC2, SC-6296 (Santa Cruz); HEB, SC-357 (Santa Cruz); LMO2, AF2726 (R&D Systems); LYL1, SC-374164 (Santa Cruz); PU.1, SC-352 (Santa Cruz); p300, SC-585 (Santa Cruz); RUNX1 (C terminus specific), ab23980 (Abcam) or IgG rabbit 12-370 (Millipore); IgG goat, SC-2346 (Santa Cruz); and IgG mouse, SC-2025 (Santa Cruz). Precipitated material was subjected to library preparation and run on an Illumina Hiseq 2000 sequencer.
Ptasinska A., Assi S.A., Martinez-Soria N., Imperato M.R., Piper J., Cauchy P., Pickin A., James S.R., Hoogenkamp M., Williamson D., Wu M., Tenen D.G., Ott S., Westhead D.R., Cockerill P.N., Heidenreich O, & Bonifer C. (2014). Identification of a Dynamic Core Transcriptional Network in t(8;21) AML that Regulates Differentiation Block and Self-Renewal. Cell Reports, 8(6), 1974-1988.
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5

ChIP-seq protocol for transcription factors

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
ChIP assays were performed as described previously (Ptasinska et al., 2012 (link)). Nuclei were essentially prepared as described previously (Lefevre et al., 2003 (link)). The following antibodies were used: C/EBPα, SC-61 (Santa Cruz Biotechnology); ETO (C terminus specific), SC-9737 (Santa Cruz); HDAC2, SC-6296 (Santa Cruz); HEB, SC-357 (Santa Cruz); LMO2, AF2726 (R&D Systems); LYL1, SC-374164 (Santa Cruz); PU.1, SC-352 (Santa Cruz); p300, SC-585 (Santa Cruz); RUNX1 (C terminus specific), ab23980 (Abcam) or IgG rabbit 12-370 (Millipore); IgG goat, SC-2346 (Santa Cruz); and IgG mouse, SC-2025 (Santa Cruz). Precipitated material was subjected to library preparation and run on an Illumina Hiseq 2000 sequencer.
Ptasinska A., Assi S.A., Martinez-Soria N., Imperato M.R., Piper J., Cauchy P., Pickin A., James S.R., Hoogenkamp M., Williamson D., Wu M., Tenen D.G., Ott S., Westhead D.R., Cockerill P.N., Heidenreich O, & Bonifer C. (2014). Identification of a Dynamic Core Transcriptional Network in t(8;21) AML that Regulates Differentiation Block and Self-Renewal. Cell Reports, 8(6), 1974-1988.
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