A competitive immunoassay was used for the determination of F2-isoprostanes in urine (Cayman Chemical, Charlotte, USA). Urine was purified using the solid phase extraction cartridges. The purification and the subsequent ELISA assay were performed following the manufacturer’s recommendations. Plasma protein carbonyls and erythrocyte glutathione were determined spectrophotometrically as described previously [17 (link)]. Oxidation of GSH was prevented using N-ethylmaleimide, which is widely considered the most appropriate blocking agent for preventing glutathione oxidation [18 (link)]. Positive controls have been used in both the ELISA and the spectrophotometric assays.
F2 isoprostanes
Manufactured by Cayman Chemical
Sourced in United States
F2-isoprostanes are a class of prostaglandin-like compounds that are formed by the free radical-catalyzed peroxidation of arachidonic acid. They serve as reliable markers of oxidative stress and lipid peroxidation in biological systems.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using f2 isoprostanes
1
Measurement of Oxidative Stress Biomarkers
2
Hepatic Biomarkers and Cytokines
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Transaminases levels, AST (aspartate transaminase) and ALT (alanine transaminase), were measured using a specific diagnostic kit (SpinReact, Girona, Spain) and expressed as international units/L. ELISA kits were used for assessment of serum levels (pg/mL) of TNF-α, IL-6, IL-1β, and IL-10 cytokines (Biosource International, Camarillo, CA, USA) and F2-isoprostanes (Cayman Chemical, Ann Arbor, MI, USA). GSH and GSSG contents (μmol/g liver) were measured using a specific glutathione assay kit (Cayman Chemical).
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