Metavue 4 6r8

Cells were grown in SM with or without 2% glucose, 100 μl of the culture was centrifuged, and 80 μl of the supernatant was removed. The cells were then resuspended in the remaining 20 μl. Portions (2 μl) of each sample were mounted on glass slides treated with 0.1% (wt/vol) poly-l-lysine (Sigma). If necessary, 4′,6-diamidino-2-phenylindole (DAPI) solution (1 mg/ml in water) was added to the cell suspension at 1 μg/ml. Microscopy was performed with an Olympus BX51 phase-contrast and fluorescence microscope with a 100× Plan-N objective (Olympus, Tokyo, Japan). Images were captured using a CoolSNAP HQ charge-coupled device camera (Nippon Roper, Tokyo, Japan) and Metavue 4.6r8 software (Universal Imaging, PA).

Cells were picked up from flesh colony on LB agar plate and inoculated to 1 ml of LB medium in L-tube. The tube was shaken for 14 h at 37°C. One hundred microliters of the culture was centrifuged, and 80 μl of the supernatant was removed. The cells were then resuspended in the remaining 20 μl. Portions (2 μl) of each sample were mounted on glass slides treated with 0.1% (wt/vol) poly-L-lysine (Sigma-Aldrich, MO, United States). Microscopy was performed with an Olympus BX51 phase contrast and fluorescence microscope with a 100 × PLAN-N objective (Olympus, Tokyo, Japan). Images were captured using a CoolSNAP HQ charge-coupled device camera (Nippon Roper, Tokyo, Japan) and Metavue 4.6r8 software (Universal Imaging, PA, United States).