Sleepaway

Manufactured by Zoetis

Sourced in United States

Sleepaway is a compact and portable laboratory equipment designed to facilitate the sleep monitoring and analysis of research animals. It provides a controlled environment for conducting sleep-related studies.

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Lab products found in correlation

Fd neurosilver, by FD NeuroTechnologies (2 mentions) Dp73 digital camera, by Olympus (2 mentions) Rnalater, by Qiagen (1 mentions) Ax70 microscope, by Olympus (1 mentions) Paraformaldehyde (pfa), by Leica Microsystems (1 mentions) Sm2000r, by Leica Microsystems (1 mentions) Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions) Sodium pentobarbital, by Zoetis (1 mentions) Isoflo, by Abbott (1 mentions) Epiquik nuclear extraction kit, by Epigentek (1 mentions)

15 protocols using sleepaway

Ethical Animal Research Procedures

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This study followed strict accordance with recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. All experimental procedures were reviewed and approved by the University of Vermont’s Institutional Animal Care and Use Committee (IACUC protocol: 14–003). All mice were euthanized by sodium pentobarbital (SleepAway, Zoetis Inc, Kalamazoo, MI, USA) overdose followed by transcardial perfusion. All efforts were made to minimize suffering.

Delay E.R., Socia S.H., Girardin J.L., Jewkes B.C., King J.H, & Delay R.J. (2019). Cyclophosphamide and the taste system: Effects of dose fractionation and amifostine on taste cell renewal. PLoS ONE, 14(4), e0214890.

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Porcine Tissue Sampling for SARS-CoV-2 Analysis

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On 2 and 10 DPI, two randomly selected animals from each inoculated group (Groups 1 and 2) and one negative control pig (Group 4) were euthanized and necropsied, with the remaining three inoculated pigs in each group euthanized and necropsied on 20 DPI (Group 4) or 21 DPI (Groups 1, 2, and contact controls).
Humane euthanasia was completed through intravenous injection of Sleepaway (Zoetis) according to the IACUC protocol. During necropsy, the following tissue samples were collected: grossly visible external lesion tissue; intradermal injection site (nasal planum); lymph nodes (mandibular, parotid, retropharyngeal, superficial cervical); tonsil; testicle; Peyer’s patch/ileum; kidney; parotid salivary gland; mammary gland; gluteal skeletal muscle; lung; spleen; urinary bladder; and any other tissue with a grossly-visible lesion. The lymph nodes tested were chosen based on their anatomic location with respect to lymphatic drainage of the inoculation site. Tissue samples were placed in RNAlater (Qiagen) for RT-qPCR analysis. For immunohistochemistry (IHC) examination, select samples were placed in 10% neutral buffered formalin for 20–30 days.

Morozov I., Monath T.P., Meekins D.A., Trujillo J.D., Sunwoo S.Y., Urbaniak K., Kim I.J., Narayanan S.K., Indran S.V., Ma W., Wilson W.C., O'Connor C., Dubey S., Troth S.P., Coller B.A., Nichols R., Martin B.K., Feldmann H, & Richt J.A. (2021). High dose of vesicular stomatitis virus-vectored Ebola virus vaccine causes vesicular disease in swine without horizontal transmission. Emerging Microbes & Infections, 10(1), 651-663.

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Brain Tissue Preparation for Immunocytochemistry

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All rats and mice in these experiments were sexed using anogenital distance as a marker. Neonatal rats were anesthetized by hypothermia, while juvenile and adult rats and mice were administered a lethal injection of sodium pentobarbital (Sleepaway; Zoetis). All animals were euthanized by decapitation, and brains were rapidly removed and immersed in 5% acrolein in 0.1M phosphate buffer (pH 7.6) for 6 hours, then cryoprotected in 30% sucrose in 0.1M phosphate buffer for 72h. Brains were sectioned in the coronal plane at 50μm on a freezing microtome and stored in cryoprotectant (30% sucrose, 0.1% polyvinyl-pyrrolidone-40 in ethylene glycol and 0.1M phosphate buffer) until immunocytochemical processing.

Willing J, & Wagner C.K. (2015). Progesterone receptor expression in the developing mesocortical dopamine pathway: importance for complex cognitive behavior in adulthood. Neuroendocrinology, 103(3-4), 207-222.

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Lung Toxicity Assessment Post Spot Welding Aerosol Exposure

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At 24 h after 3, 8 and 13 d (4 h/d) of spot welding aerosol exposure, separate sets of rats were deeply anesthetized with an overdose of Sleepaway (26% sodium pentobarbital, 7.8% isopropyl alcohol and 20.7% propylene glycol; ip) (Fort Dodge Animal Health, Fort Dodge, IA). Blood samples were collected via the vena cava, then the abdominal aorta was cut to exsanguinate the rat. The left bronchus was clamped and bronchoalveolar lavage (BAL) was done on the right lung lobes with Ca²⁺ and Mg²⁺-free phosphate buffered saline (PBS). The right lung was lavaged with 4 ml of PBS, kept separate on ice, followed by four washes (5 ml/wash). Both lavage fractions were then centrifuged (500g, 10 min, 4 °C) and the acellular supernatant of the first lavage used for evaluation of lung toxicity (below). Finally, the cell pellets of the first and subsequent washes were combined then suspended in 1 ml PBS for cell counts and differentials.

Zeidler-Erdely P.C., Meighan T.G., Erdely A., Fedan J.S., Thompson J.A., Bilgesu S., Waugh S., Anderson S., Marshall N.B., Afshari A., McKinney W., Frazer D.G, & Antonini J.M. (2014). Effects of acute inhalation of aerosols generated during resistance spot welding with mild-steel on pulmonary, vascular and immune responses in rats. Inhalation toxicology, 26(12), 697-707.

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Bleomycin-Induced Lung Injury Assessment

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Animals were euthanized by i.p. injection of Sleepaway (2 mL/kg, Fort Dodge Animal Health, Fort Dodge, IA) 3 d, 7 d or 21 d post saline control or bleomycin. Anti-TNFα antibody treated study groups were euthanized at 7 d and 21 d post bleomycin. BAL was collected by slowly instilling and withdrawing (x 5) 1 mL of ice-cold saline into the lung through a cannula inserted into the trachea. BAL was centrifuged (300 g, 8 min), and supernatants analyzed for protein and phospholipid. Cell- free supernatants were assayed for protein content using a BCA protein assay kit (Pierce Biotechnologies Inc., Rockford, IL) with bovine serum albumin as the standard. Cell pellets were resuspended in 500 μL of PBS, enumerated using a Beckman Coulter counter (Indianapolis, IN), and processed for flow cytometric analysis.

Venosa A., Gow J.G., Taylor S., Golden T.N., Murray A., Abramova E., Malaviya R., Laskin D.L, & Gow A.J. (2021). Myeloid cell dynamics in bleomycin-induced pulmonary injury in mice; effects of anti-TNFα antibody. Toxicology and applied pharmacology, 417, 115470.

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Neuroinflammation and Neurodegeneration Analysis

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Mice were anesthetized with Sleep Away^® (0.1 mL; Fort Dodge Animal Health, Fort Dodge, IA, USA) and transcardially perfused with saline (0.9%) followed by 4% paraformaldehyde in 0.1 M PBS 24 h post DFP injection. Brains were removed and stored in 4% paraformaldehyde in 0.1 M PBS to be sent to FD Neurotechnologies (Columbia, MD, USA) for subsequent processing and staining for GFAP and ionized calcium-binding adapter molecule 1 (Iba1) (markers for activation of astrocytes and microglia, respectively), as well as FD NeuroSilver™ and Fluoro-Jade B (markers for neuronal degeneration). The sections were visualized using an Olympus AX70 microscope (Olympus, Pittsburgh, PA, USA) with an UPlanFl 4× 0.13 numerical aperture (GFAP, Iba1, and Fluoro-Jade B) or 10× 0.30 numerical aperture (FD NeuroSilver™) objective lens and images captured using Cell Sens Dimension software with the Olympus DP73 digital camera (Olympus, Pittsburgh, PA, USA) attached to the microscope. Post-processing included cropping images to isolate the HIP.

O'Callaghan J.P., Kelly K.A., Locker A.R., Miller D.B, & Lasley S.M. (2015). Corticosterone primes the neuroinflammatory response to DFP in mice: potential animal model of Gulf War Illness. Journal of Neurochemistry, 133(5), 708-721.

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Liver Ablation Lesion Histopathology

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No animal died after the surgery. At 7 days after the ablation, all the animals were euthanized with an overdose of intravenous pentobarbital sodium (120 mg/kg, Sleepaway; Fort Dodge Animal Health, Fort Dodge, IA). The liver tissues from the ablation tract to the gallbladder were harvested, and the ablation lesions were cut and sectioned from the center. The maximum diameter was measured, and the lesions were photographed. Then, the t issue samples, including the abnormal and adjacent normal tissues, were fixed in 10% neutral buffered formalin, processed routinely for histology, embedded in paraffin, cut into 5-μm-thick slices and stained with hematoxylin and eosin. Pathological analyses were performed by an attending surgical pathologist.

Zeng J., Qin Z., Zhou L., Fang G., Chen J., Li J., Niu L., Liang B, & Xu K. (2017). Comparison between cryoablation and irreversible electroporation of rabbit livers at a location close to the gallbladder. Radiology and Oncology, 51(1), 40-46.

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Bronchoalveolar Lavage Analysis of Lung

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Animals were euthanized 3 d after exposure to NM or PBS by i.p. injection of Sleepaway® (0.44 kg/ml; Fort Dodge Animal Health, Fort Dodge, IA). PBS (10 ml) was instilled into the lung via a cannula in the trachea. BAL was collected by slowly withdrawing the fluid. BAL fluid was centrifuged (300 x g, 8 min), supernatants collected, aliquoted, and stored at −80°C until analysis. The cell pellet was resuspended in 1 ml PBS and viable cells counted with a hemocytometer using trypan blue dye exclusion. Total protein was quantified in cell-free BAL using a BCA Protein Assay kit (Pierce Biotechnologies Inc., Rockford, IL) with bovine serum albumin (BSA) as the standard.

Sunil V.R., Vayas K.N., Cervelli J.A., Malaviya R., Hall L., Massa C.B., Gow A.J., Laskin J.D, & Laskin D.L. (2014). Pentoxifylline Attenuates Nitrogen Mustard-induced Acute Lung Injury, Oxidative Stress and Inflammation. Experimental and molecular pathology, 97(1), 89-98.

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Rat Brain Perfusion and Sectioning

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The day following the completion of behavioral testing all rats were deeply anesthetized (Sleep-Away, Fort Dodge Animal Health, Fort Dodge, IA) and then transcardially perfused with ice-cold (4°C) phosphate buffered saline (PBS) followed by ice-cold (4°C) filtered 4% paraformaldehyde (Electron Microscopy Services, Hatfield, PA). Whole brains were extracted, kept in paraformaldehyde (4%) for 24-hr, post-fixed in a 30% sucrose solution made in 0.1M PBS and coronally sliced at 40 µm on a freezing sliding microtome (Sm2000r; Leica Instruments, Wetzler, Germany). Tissue sections were sequentially collected individually in a 96 well plate containing a cryoprotectant solution (62.8 mg NaH₂PO₄, 2.18 g Na₂HPO₄, 160 mL dH₂O, 120 mL ethylene glycol and 120 mL glycerol; pH: 7.4) and stored at −20°C until immunohistochemistry. All tissues were run in cohorts that include subjects from all treatment conditions to control for specimen preparation procedures.

Hall J.M, & Savage L.M. (2016). Exercise leads to the re-emergence of the cholinergic/nestin neuronal phenotype within the medial septum/diagonal band and subsequent rescue of both hippocampal ACh efflux and spatial behavior. Experimental neurology, 278, 62-75.

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Oral Nicotine Exposure in Mice

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The experimental design used for the study is shown in Figure 1. Twenty-one mice were divided randomly into three groups of seven mice each and each mouse was treated (via the oral mucosa) for 3 week (5 day/week, once/d) in the same manner with either: (1) equal volumes (50 μL) of water (control); (2) a solution of nicotine dissolved in water, containing 0.24 mg of nicotine; (3) or the gutkha solution as described above. Each mouse was weighed daily prior to treatment. Blood was collected (via tail bleed) weekly from a subset of each treatment group (3 mice/group) approximately 1 h after exposure and serum cotinine measured using an EIA kit according to the manufacturer’s instructions (OraSure Technologies, Inc., Bethlehem, PA, USA). All mice were euthanized 24 h after the last exposure by ip injection of pentobarbital (0.2 mL of Sleepaway (Fort Dodge Animal Health, Fort Dodge, IA, USA) diluted 1:10 in PBS) and blood collected from the descending aorta. Heart, testes, liver and tongue were weighed and the liver quickly frozen in liquid nitrogen and stored at −80 °C for later analysis of gene expression.

Willis D.N., Popovech M.A., Gany F., Hoffman C., Blum J.L, & Zelikoff J.T. (2014). Toxicity of Gutkha, a Smokeless Tobacco Product Gone Global: Is There More to the Toxicity than Nicotine?. International Journal of Environmental Research and Public Health, 11(1), 919-933.

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