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4 6 diamidino 2 pheylindole dapi

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4',6-diamidino-2-pheylindole (DAPI) is a fluorescent stain that binds to DNA. It is commonly used in fluorescence microscopy to visualize and study the structure of cell nuclei.

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Lab products found in correlation

Bovine serum albumin (bsa), by Santa Cruz Biotechnology (1 mentions) Alexa flour 488 dye conjugated secondary antibodies, by Thermo Fisher Scientific (1 mentions) Nunc lab tek 2 chamber slide system, by Thermo Fisher Scientific (1 mentions) Hpa001122, by Merck Group (1 mentions)

2 protocols using 4 6 diamidino 2 pheylindole dapi

1

Immunocytochemical Analysis of Stem Cell Markers

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For immunocytochemistry (ICC) analysis, both 682, 779, 464T, and 532T cells (3×103 cells/well) were cultured on Nunc Lab-Tek II Chamber Slide System (Thermo Scientific, Waltham, MA, USA). The cells were fixed by 4% paraformaldehyde, permeabilized in 0.5% Triton X-100, and then blocked with 1% bovine serum albumin (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Primary antibodies against Sox2 and nestin (Upstate/Millipore, Billerica, MA, USA) and Alexa-flour 488 dye-conjugated secondary antibodies (Invitrogen) were utilized. Nuclei were stained by 4',6-diamidino-2-pheylindole (DAPI, Invitrogen).
Woo S.R., Oh Y.T., An J.Y., Kang B.G., Nam D.H, & Joo K.M. (2015). Glioblastoma specific antigens, GD2 and CD90, are not involved in cancer stemness. Anatomy & Cell Biology, 48(1), 44-53.
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2

Immunofluorescence Staining for WDHD1

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Cells were fixed in 4% PBS-paraformaldehyde for 15 min, incubated in 0.1% Triton-X-100 for 5 min on ice, then in 0.2% Fish Skin Gelatine in PBS for 1 h and stained for 1 h with an anti-WDHD1 (1:500, Sigma-Aldrich, HPA001122). Protein expression was detected using Alexa Fluor (1:400, Molecular Probes) for 20 min. 4'6-Diamidino-2-Pheylindole (DAPI) (Invitrogen) was used to stain nuclei (1:1000). Samples were observed using a confocal microscope system (Leica SP8). Acquired images were analysed using Fiji58 (link).
Ertay A., Liu H., Liu D., Peng P., Hill C., Xiong H., Hancock D., Yuan X., Przewloka M.R., Coldwell M., Howell M., Skipp P., Ewing R.M., Downward J, & Wang Y. (2020). WDHD1 is essential for the survival of PTEN-inactive triple-negative breast cancer. Cell Death & Disease, 11(11), 1001.
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