Cells were grown on tissue-treated 8-well chambered coverslides (Ibidi, Martinsried, Germany), cultured, and fixed as described before [56 (link)]. DAPI (4′,6-diamidine-2′-phenylindole dihydrochloride) solution (Roche Diagnostics, Mannheim, Germany) and phalloidin Alexa Fluor 488 solution (Thermo Fisher) was applied for 1 h. Confocal laser scanning microscopy was performed with a Zeiss LSM510 Meta system equipped with an inverted Observer Z1 microscope and a Plan-Apochromat 63 ×/1.4 oil immersion objective (Carl Zeiss MicroImaging GmbH, Göttingen, Germany).
8 well chambered cover slides
Manufactured by Ibidi
Sourced in Germany
The 8-well chambered cover slides are a laboratory equipment designed for cell culture applications. Each slide features eight individual chambers that allow for the simultaneous analysis of multiple samples or experimental conditions. The slides are made of high-quality materials and are intended to provide a controlled environment for cell growth and observation.
Automatically generated - may contain errors
Lab products found in correlation
Plan apochromat 63 1.4 oil immersion objective, by Zeiss (2 mentions)
Lsm 510 meta system, by Zeiss (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle medium, by Thermo Fisher Scientific (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Roche (1 mentions)
Observer z1 microscope, by Zeiss (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Ki67 sp6, by Abcam (1 mentions)
4 protocols using 8 well chambered cover slides
1
Immunofluorescence Imaging of Cells
2
Dendritic Cell Microscopy Protocol
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Tissue-treated 8-well chambered cover slides (Ibidi, Martinsried, Germany) were used for dendritic cells growth. The cells were cultured and fixed as previously described [19 (link)]. DAPI (4′,6-Diamidine-2′-phenylindole dihydrochloride) solution (Roche Diagnostics, Mannheim, Germany) and phalloidin Alexa Flour 488 solution (Thermo Fisher) were applied for 1 h. A Zeiss LSM510 Meta system equipped with an inverted Observer Z1 microscope and a Plan-Apochromat 63 × /1.4 oil immersion objective (Carl Zeiss MicroImaging GmbH, Göttingen, Germany) were used for confocal laser scanning microscopy.
3
Culturing BSC-01 Cells for Experiments
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BSC-01 cells were cultured in phenol red-free Dulbecco’s modified Eagle medium (Thermo Fisher), supplemented with 1 mM sodium pyruvate (Thermo Fisher) and 10% fetal bovine serum (Thermo Fisher), at 37 °C in a humidified 5% CO2 atmosphere. The cells were seeded into 8-well chambered cover slides (ibidi GmbH) and used 2 d after seeding.
4
Immunofluorescence Microscopy of SGPL1 and FOXO3a
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Cells were grown on tissue-treated 8-well chambered cover slides (Ibidi, Martinsried, Germany) and fixed as described before in Ref. [11 (link)] Primary SGPL1 antibody SGPL1 (HPA021125, Atlas Antibodies), FOXO3a (D19A7, Cell Signaling), Ki-67 (SP6, Abcam), as well as secondary anti-rabbit IgG (GE Healthcare) and DAPI (4′,6-Diamidine-2′-phenylindole dihydrochloride, Roche Diagnostics, Mannheim, Germany), were used. Confocal laser scanning microscopy was performed with a Zeiss LSM510 Meta system equipped with an inverted Observer Z1 microscope and a Plan-Apochromat 63×/1.4 or 40× oil immersion or 20× objective (Carl Zeiss MicroImaging GmbH, Göttingen, Germany).
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