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Lightning link rapid atto565

Manufactured by Abcam
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The Lightning Link Rapid Atto565 is a labeling reagent that enables rapid and efficient conjugation of antibodies or other proteins with the Atto565 fluorescent dye. The product provides a straightforward and user-friendly conjugation process without the need for purification steps.

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Lab products found in correlation

Irdye800cw donkey anti mouse igg h l, by LI COR (2 mentions) Goat anti mouse af647, by Thermo Fisher Scientific (2 mentions) Goat anti rabbit af488, by Thermo Fisher Scientific (2 mentions) Dylight 488, by Abcam (2 mentions) Atto 565, by Abcam (2 mentions) Tub 1a2, by Merck Group (2 mentions) Irdye680rd donkey anti rabbit igg h l, by LI COR (2 mentions)

2 protocols using lightning link rapid atto565

1

Comprehensive Tubulin Cytoskeleton Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Alpha tubulin; mouse monoclonal, clone DM1A (Cell Signaling #3873)- isolated myocyte IF 1:100, tissue section IF 1:50 (primary labeled Atto- or dylight-488, Innova Biosciences, 322–0010, 350–0010); WB 1:1000
Tyrosinated tubulin; mouse monoclonal, clone TUB-1A2 (Sigma T 9028); isolated myocyte IF 1:200, WB 1:1000
Detyrosinated tubulin; rabbit polyclonal (abcam ab48389); isolated myocyte IF 1:200; WB 1:1000
Tubulin tyrosine ligase; rabbit polyclonal (proteintech 13618–1-AP); WB 1:500
GAPDH; mouse monoclonal, clone 3B1E9 (GenScript A01622–40); WB 1:1000
Desmin; goat polyclonal (R&D AF3844); IF 1:100 (primary labeled Atto 565, Innova Biosciences); WB: 1:1000
Goat anti-mouse AF 647 (Life Technologies, A-21235); IF 1:500
Goat anti-rabbit AF 488 (Life Technologies, A-11034); IF 1:500
IRDye800CW Donkey anti-Mouse IgG (H + L) (LI-COR, # 925–32212); WB 1:10000
IRDye680RD Donkey anti-Rabbit IgG (H + L) (LI-COR, #925–68073); WB 1:10000
Lightning Link Rapid Atto565 (Innova Biosciences, 351–0030), Atto 488 (322–0010) and dylight 488 (350–0010)
Hoechst 33342 (1:1000) for labeling nuclei
Yingxian Chen C., Caporizzo M.A., Bedi K., Vite A., Bogush A.I., Robison P., Heffler J.G., Salomon A.K., Kelly N.A., Babu A., Morley M.P., Margulies K.B, & Prosser B.L. (2018). Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure. Nature medicine, 24(8), 1225-1233.
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2

Comprehensive Tubulin Cytoskeleton Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Alpha tubulin; mouse monoclonal, clone DM1A (Cell Signaling #3873)- isolated myocyte IF 1:100, tissue section IF 1:50 (primary labeled Atto- or dylight-488, Innova Biosciences, 322–0010, 350–0010); WB 1:1000
Tyrosinated tubulin; mouse monoclonal, clone TUB-1A2 (Sigma T 9028); isolated myocyte IF 1:200, WB 1:1000
Detyrosinated tubulin; rabbit polyclonal (abcam ab48389); isolated myocyte IF 1:200; WB 1:1000
Tubulin tyrosine ligase; rabbit polyclonal (proteintech 13618–1-AP); WB 1:500
GAPDH; mouse monoclonal, clone 3B1E9 (GenScript A01622–40); WB 1:1000
Desmin; goat polyclonal (R&D AF3844); IF 1:100 (primary labeled Atto 565, Innova Biosciences); WB: 1:1000
Goat anti-mouse AF 647 (Life Technologies, A-21235); IF 1:500
Goat anti-rabbit AF 488 (Life Technologies, A-11034); IF 1:500
IRDye800CW Donkey anti-Mouse IgG (H + L) (LI-COR, # 925–32212); WB 1:10000
IRDye680RD Donkey anti-Rabbit IgG (H + L) (LI-COR, #925–68073); WB 1:10000
Lightning Link Rapid Atto565 (Innova Biosciences, 351–0030), Atto 488 (322–0010) and dylight 488 (350–0010)
Hoechst 33342 (1:1000) for labeling nuclei
Yingxian Chen C., Caporizzo M.A., Bedi K., Vite A., Bogush A.I., Robison P., Heffler J.G., Salomon A.K., Kelly N.A., Babu A., Morley M.P., Margulies K.B, & Prosser B.L. (2018). Suppression of detyrosinated microtubules improves cardiomyocyte function in human heart failure. Nature medicine, 24(8), 1225-1233.
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