Polyvinylidene difluoride pvdf

Manufactured by GE Healthcare

Sourced in Germany, United Kingdom

Polyvinylidene difluoride (PVDF) is a type of lab equipment used in various analytical and research applications. It is a synthetic polymer material with unique physical and chemical properties. PVDF is commonly used as a membrane or filter material in various laboratory techniques, such as Western blotting, immunoassays, and sample preparation.

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Hybond®-LFP polyvinylidene difluoride (PVDF) membranes Polyvinylidene difluoride (PVDF) membrane Polyvinylidene difluoride (PVDF) membranes (Hybond-P) 0.45‐μm polyvinylidene difluoride (PVDF) membrane Hybond™-P polyvinylidene difluoride (PVDF) blotting membranes Polyvinylidene difluoride (PVDF) blotting membrane Hybond-P polyvinylidene difluoride (PVDF) membrane PVDF (polyvinylidene difluoride) membranes Amersham Hybond-P polyvinylidene difluoride (PVDF) membranes Polyvinylidene difluoride

3 protocols using polyvinylidene difluoride pvdf

Mass Spectrometry-Based Protein Analysis

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Bovine serum albumin fraction V (BSA; catalogue n. A6588), amino acids (catalogue n. LAA21), dopamine (catalogue n. H8502), acetylcholine (catalogue n. A6625) tyramine (catalogue n. T2879), γ-aminobutyric acid (GABA, catalogue n. 03,835), DL-lysine-4,4,5,5-d₄ dihydrochloride (d4-lysine, catalogue n. 489,034), formic acid (catalogue n. 5.33002), salts, and buffers were purchased from Sigma-Aldrich (St. Louis, MO, USA). Nε-carboxymethyllysine (CML, catalogue n. HAA2950) and Nε-carboxyethyllysine (CEL, catalogue n. HAA2940) were obtained from Iris Biotech (Marktredwitz, Germany). Acetonitrile (catalogue n. 1.00029), water (catalogue n. 1.15333), and ammonium formate (catalogue n. 70,221) of mass spectrometry grade were obtained from Merck (Darmstadt, Germany).
Polyvinylidene difluoride (PVDF; catalogue n. GEH10600021) and nitrocellulose membranes (catalogue n GEH10600001) were from GE Healthcare (Milan, Italy).
Fuji Super RX films (catalogue n. 47,410–19,284), FujiFilm Man-X Developer (catalogue n. 949–966) and FujiFilm Man-X Fixer (catalogue n. 949–974) were from Laboratorio Elettronico Di Precisione (Naples, Italy).

Spagnuolo M.S., Mazzoli A., Nazzaro M., Troise A.D., Gatto C., Tonini C., Colardo M., Segatto M., Scaloni A., Pallottini V., Iossa S, & Cigliano L. (2022). Long-Lasting Impact of Sugar Intake on Neurotrophins and Neurotransmitters from Adolescence to Young Adulthood in Rat Frontal Cortex. Molecular Neurobiology, 60(2), 1004-1020.

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Isolation and Characterization of Novel Cadalene Derivatives

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RPMI 1640 medium, gentamicin, trypsin, phosphate-buffered saline (PBS) and fetal bovine serum (FBS) were acquired from Gibco (Invitrogen, Carlsbad, CA, USA). Isolated compounds 7-hydroxy-3,4-dihydrocadalene (>98.0%), 7-(phenylcarbamate)-3,4-dihydrocadalene (77% yield) and 7-(phenylcarbamate)-cadalene (79% yield) were generously provided by Dr. Guillermo Delgado Lamas (Instituto de Química, UNAM). Their isolation or preparation and structural characterization are described by Egas et al. (2017) (link).
Dimethyl Sulphoxide (DMSO), H₂O₂, 2′,7′-Dichlorofluorescein Diacetate (DCFH-DA), 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), Bovine Serum Albumin (BSA), 2-thiobarbituric acid, antimycin A, oligomycin, rotenone, RIPA buffer, phosphatase inhibitor cocktails, bicinchoninic acid reagent, Tris Buffered Saline-Tween 20, GAPDH antibody (G-8795), caspase 3 (sc-7148) and Bcl-2 (sc-492) antibodies were purchased from Sigma-Aldrich (St. Louis, MO, USA). Protease inhibitor cocktail from Roche (Lewes, UK). Polyvinylidene difluoride (PVDF) membranes from GE Healthcare (Buckinghamshire, UK).

Mendoza-Fuentes A., González-Burgos E., Aparicio Trejo O.E., Delgado-Lamas G., Rodríguez-Chávez J.L., Pedraza-Chaverri J., Gómez-Serranillos M.P, & Araiza-Olivera D. (2023). The cytotoxicity effect of 7-hydroxy-3,4-dihydrocadalene from Heterotheca inuloides and semisynthetic cadalenes derivates towards breast cancer cells: involvement of oxidative stress-mediated apoptosis. PeerJ, 11, e15586.

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Protein Extraction and Western Blot Analysis

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Proteins were extracted using ice-cold lysis buffer (50 mM Tris-HCl pH 7.5, 120 mM NaCl, 20 mM NaF, 1 mM EDTA, 6 mM EGTA, 15 mM Na-pyrophosphate, 0.5 mM Na-orthovanadate, 1 mM benzamidine, 0.1 mM phenylmethylsulfonyl fluoride and 1% Nonidet P-40). Protein concentration was determined using the Bio-Rad Protein Assay Reagent (Bio-Rad). Proteins were separated on SDS-polyacrylamide gels, transferred to polyvinylidene difluoride (PVDF) (GE-Healthcare) and probed with appropriate antibodies. Immunoblot analysis was performed using BioRad Clarity™ Western Substrate (#170-5060) and proteins were visualized with the FUSION SOLOÒ chemiluminescence imaging system (Vilber) [25] . Immunoprecipitation was performed as described previously [24] . To ensure that the observed interactions were not DNAmediated, ethidium bromide was included in all samples during immunoprecipitation.

Eid W., Hess D., König C., Gentili C, & Ferrari S. (2019). The human Exonuclease-1 interactome and phosphorylation sites. Biochemical and biophysical research communications, 514(3).

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