Cathepsin B activity was measured using a Magic Red Cathepsin B assay kit (Bio-Rad) according to the manufacturer’s instructions. Images were captured with the integrated Columbus image data storage and analysis system.
Magic red cathepsin b assay kit
Manufactured by Bio-Rad
The Magic Red Cathepsin B assay kit is a fluorescence-based detection system used to measure the activity of the lysosomal protease Cathepsin B in cell and tissue samples. The kit utilizes a cell-permeable, fluorogenic substrate that becomes fluorescent upon cleavage by Cathepsin B, enabling quantitative assessment of its enzymatic activity.
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Lab products found in correlation
2 protocols using magic red cathepsin b assay kit
1
Cathepsin B Activity Measurement
2
Cathepsin B Degradation Assay
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Magic Red Cathepsin B assay kit (Bio-Rad) was used to determine the cathepsin B-mediated degradative capacity of BMDM exposed to compounds of interest. The experiments were performed as described for the LysoTracker labeling except that the Magic Red Cathepsin B assay kit was used in accordance with the manufacturer’s instructions.
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