Anti g3pdh human polyclonal antibody

On days 0, 1, 3, and 5 postinfection “Arlo” cells were lysed for Western blot analysis using Triton‐X‐100 sample buffer, as described previously.^[130] Proteins were separated by SDS‐PAGE. Specific antibodies against SARS‐CoV‐2 N (1:1000 dilution, SARS‐CoV‐2 Nucleocapsid Antibody, Rabbit monoclonal antibody (Mab), #40143‐R019, Sino Biological), ACE2 (1:500 dilution, Anti‐ACE2 antibody, #ab15348, Abcam), TMPRSS2 (1:1000 dilution, Recombinant Anti‐TMPRSS2 antibody [EPR3861], #ab92323, Abcam), and GAPDH (1:1000 dilution, Anti‐G3PDH Human Polyclonal Antibody, #2275‐PC‐100, Trevigen) allowed antigen detection. Protein bands were made visible by laser‐induced fluorescence using an infrared scanner for protein quantification (Odyssey, Li‐Cor Biosciences).

The cells were lysed using a Triton-X-100 sample buffer, and the proteins were separated by SDS-PAGE. Detection occurred using specific antibodies against CD47 (1:100 dilution, CD47 Antibody, anti-human, Biotin, REAfinity™, # 130-101-343, Miltenyi Biotec, Bergisch Gladbach, Germany), SARS-CoV-2 N (1:1000 dilution, SARS-CoV-2 Nucleocapsid Antibody, Rabbit MAb, #40143-R019, Sino Biological, Beijing, China), SIRPα (1:1000 dilution, SIRPα/SHPS1 (D6I3M) Rabbit mAb #13379, Cell Signaling, Danvers, MA, USA), and GAPDH (1:1000 dilution, Anti-G3PDH Human Polyclonal Antibody, #2275-PC-100, Trevigen, Gaithersburg, MD, USA). Protein bands were visualized and quantified by laser-induced fluorescence using an infrared scanner for protein quantification (Odyssey, Li-Cor Biosciences, Bad Homburg vor der Höhe, Germany).

Cells were lysed using Triton-X-100 sample buffer (Sigma-Aldrich), and proteins were separated by SDS-PAGE. Detection occurred by using specific antibodies against SARS-CoV-2 N (1:1000 dilution, SARS-CoV-2 Nucleocapsid Antibody, Rabbit monoclonal antibody (Mab), #40143-R019, Sino Biological), ACE2 (1:500 dilution, Anti-ACE2 antibody, #ab15348, Abcam, Berlin, Germany), TMPRSS2 (1:1000 dilution, Recombinant Anti-TMPRSS2 antibody [EPR3861], #ab92323, Abcam), and GAPDH (1:1000 dilution, Anti-G3PDH Human Polyclonal Antibody, #2275-PC-100, Trevigen, Wiesbaden, Germany). Protein bands were visualized by laser-induced fluorescence using an infrared scanner for protein quantification (Odyssey, Li-Cor Biosciences, Bad Homburg, Germany).