Cell apoptosis was determined using an Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) apoptosis detection kit (Beyotime, Shanghai, China). After the indicated treatments, the cells were collected through trypsinization, followed by washing twice with PBS. For analysis of apoptosis, the human lens epithelial cells (HLECs) were stained with Annexin V-FITC for 15 min at room temperature and then incubated with PI on ice. After incubation, all samples were analyzed by flow cytometry (BD Biosciences, Franklin Lakes, NJ, USA). Cells stained positive with Annexin V and negative with PI were considered as early apoptosis.
Annexin 5 fluorescein isothiocyanate fitc and propidium iodide apoptosis detection kit
Manufactured by Beyotime
Sourced in China
The Annexin V-FITC and propidium iodide (PI) apoptosis detection kit is a laboratory tool used to detect and quantify apoptosis, a form of programmed cell death. The kit utilizes Annexin V, a calcium-dependent phospholipid-binding protein that has a high affinity for phosphatidylserine, a marker of apoptosis. FITC-labeled Annexin V and PI are used to distinguish between early apoptotic, late apoptotic, and necrotic cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using annexin 5 fluorescein isothiocyanate fitc and propidium iodide apoptosis detection kit
1
Annexin V-FITC and PI Apoptosis Assay
2
Annexin V-FITC Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
After transfection with hsa-miR-135b-5p inhibitors or the negative control, the cells were collected in a flow tube, washed with phosphate-buffered saline (PBS), and then centrifuged. Cell apoptosis was assessed using an Annexin V/fluorescein isothiocyanate (FITC) and propidium iodide (PI) apoptosis detection kit (C1062L; Beyotime) according to the manufacturer's instructions. Annexin V-FITC, PI, and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid buffer were mixed at a ratio of 1 : 2 : 5 to make a dye liquor, of which 100 μL was used to resuspend 1 × 106 cells. Cell apoptosis was analyzed using FlowJo software. Each experiment was performed in triplicate.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!