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Ccr7 bv421 clone g043h7

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The CCR7–BV421 (clone G043H7) is a fluorescent-labeled antibody that targets the CCR7 (C-C chemokine receptor type 7) surface marker. This marker is expressed on various immune cell types and plays a role in lymphocyte trafficking and homing.

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Lab products found in correlation

Cd14 magnetic microbeads, by Miltenyi Biotec (2 mentions) Cd19 pe cy7 clone sj25c1, by Beckman Coulter (2 mentions) Cd4 pe texasred clone s3.5, by Thermo Fisher Scientific (2 mentions) Alexa fluor647 conjugated goat anti human igg, by BioLegend (2 mentions) Facsaria 3, by BD (2 mentions) Fixable viability dye, by BioLegend (1 mentions) Filipin complex from streptomyces filipinensis, by Merck Group (1 mentions) Onecomp ebeads, by Thermo Fisher Scientific (1 mentions) Cd27 apc clone m t271, by BioLegend (1 mentions) Ctb fitc, by Merck Group (1 mentions) Cd8 bv711 clone rpa t8, by BioLegend (1 mentions) Cd127 pecy7 clone a019d5, by BioLegend (1 mentions) Cd3 bv785 clone okt3, by BioLegend (1 mentions) Cd25 pe clone m a251, by BioLegend (1 mentions) Brilliant stain buffer, by BD (1 mentions) Lsrfortessa x 20 cytometer, by BD (1 mentions)

Close spelling variants of this product

CCR7 BV421‐A (clone G043H7) CCR7 (clone G043H7, CCR7 (G043H7, CCR7-BV421 Clone G043H7

3 protocols using ccr7 bv421 clone g043h7

1

Purification of Memory Immune Cells

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Monocytes were isolated from PBMCs by positive selection using CD14
magnetic microbeads (Miltenyi Biotech). CD14-depleted fractions were stained on
ice for 15–20 min with the following fluorochrome-labeled mouse
monoclonal antibodies: CD8-PE–Cy5 (clone B9.11; cat. no. A07758),
CD14-PE–Cy5 (clone RMO52; cat. no. A07765), CD16-PE–Cy5 (clone
3G8; cat. no. A07767), CD45RA-FITC (clone ALB11; cat. no. A07786) from Beckman
Coulter, CD19-PE–Cy7 (clone SJ25C1; cat. no. 341113), CD25–PE
(clone M-A251; cat. no. 555432) from BD Biosciences, CD4–PE–Texas
Red (clone S3.5; cat. no. MHCD0417) from ThermoFisher Scientific,
CCR7–BV421 (clone G043H7; cat. no. 353208) from BioLegend, Alexa Fluor
647-conjugated goat anti-human IgG (cat. no. 109-606-170) from Jackson
ImmunoResearch. IgG+ memory B cells were sorted from CD19+B cells to over 98% purity on a FACSAria III (BD). Memory CD4+ T
cells were sorted to over 98% purity after exclusion of naïve
CD4+ T cells (CD45RA+CCR7+) and
CD8+, CD14+, CD16+, CD19+,
CD25bright cells.
Cassotta A., Mikol V., Bertrand T., Pouzieux S., Le Parc J., Ferrari P., Dumas J., Auer M., Deisenhammer F., Gastaldi M., Franciotta D., Silacci-Fregni C., Fernandez Rodriguez B., Giacchetto-Sasselli I., Foglierini M., Jarrossay D., Geiger R., Sallusto F., Lanzavecchia A, & Piccoli L. (2019). A single T cell epitope drives the neutralizing anti-drug antibody response to natalizumab in multiple sclerosis patients. Nature medicine, 25(9), 1402-1407.
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2

Comprehensive Flow Cytometry Immune Profiling

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Flow cytometry staining was performed as previously described (57 (link)–59 (link)). In brief, 1 × 106 PBMCs were stained with Zombie (BioLegend) fixable viability dye for 30 min at 4°C, then labeled with antibodies to surface markers in Brilliant Stain buffer (BD Biosciences) for 30 min at 4°C. Subsequently samples were stained with 25 μg/mL cholera toxin B subunit FITC conjugate (CTB-FITC) (Sigma-Aldrich), fixed for 1 h in 2% paraformaldehyde, and stained for 2 h with 50 μg/mL filipin complex from Streptomyces filipinensis (Sigma-Aldrich) before reading the samples on a BD LSRFortessa X-20 cytometer using BD FACSDiva software. Compensation was performed using anti-mouse IgGκ/negative control compensation particles set (BD Biosciences) or OneComp eBeads (ThermoFisher Scientific), with the exception of viability dyes and filipin which were performed with single stained and unstained cells. Data was analyzed using FlowJo (Tree Star).
Antibodies for surface markers: CD45RA-BUV737 (clone HI100, BD Biosciences, 584442) CD27–APC (clone M-T271, BioLegend, 356409), CD4-AF700 (clone OKT4, eBioscience, 56-0048-82), CCR7-BV421 (clone G043H7, BioLegend, 353207), CD69-BV510 (clone FN50, BioLegend, 310936), CD8-BV711 (clone RPA-T8, BioLegend, 301044), CD3-BV785 (clone OKT3, BioLegend, 317330), CD25-PE (clone M-A251, BioLegend, 356104), CD127-PE-Cy7 (clone A019D5, BioLegend, 351320).
Waddington K.E., Papadaki A., Coelewij L., Adriani M., Nytrova P., Kubala Havrdova E., Fogdell-Hahn A., Farrell R., Dönnes P., Pineda-Torra I, & Jury E.C. (2020). Using Serum Metabolomics to Predict Development of Anti-drug Antibodies in Multiple Sclerosis Patients Treated With IFNβ. Frontiers in Immunology, 11, 1527.
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3

Purification of Memory Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Monocytes were isolated from PBMCs by positive selection using CD14
magnetic microbeads (Miltenyi Biotech). CD14-depleted fractions were stained on
ice for 15–20 min with the following fluorochrome-labeled mouse
monoclonal antibodies: CD8-PE–Cy5 (clone B9.11; cat. no. A07758),
CD14-PE–Cy5 (clone RMO52; cat. no. A07765), CD16-PE–Cy5 (clone
3G8; cat. no. A07767), CD45RA-FITC (clone ALB11; cat. no. A07786) from Beckman
Coulter, CD19-PE–Cy7 (clone SJ25C1; cat. no. 341113), CD25–PE
(clone M-A251; cat. no. 555432) from BD Biosciences, CD4–PE–Texas
Red (clone S3.5; cat. no. MHCD0417) from ThermoFisher Scientific,
CCR7–BV421 (clone G043H7; cat. no. 353208) from BioLegend, Alexa Fluor
647-conjugated goat anti-human IgG (cat. no. 109-606-170) from Jackson
ImmunoResearch. IgG+ memory B cells were sorted from CD19+B cells to over 98% purity on a FACSAria III (BD). Memory CD4+ T
cells were sorted to over 98% purity after exclusion of naïve
CD4+ T cells (CD45RA+CCR7+) and
CD8+, CD14+, CD16+, CD19+,
CD25bright cells.
Cassotta A., Mikol V., Bertrand T., Pouzieux S., Le Parc J., Ferrari P., Dumas J., Auer M., Deisenhammer F., Gastaldi M., Franciotta D., Silacci-Fregni C., Fernandez Rodriguez B., Giacchetto-Sasselli I., Foglierini M., Jarrossay D., Geiger R., Sallusto F., Lanzavecchia A, & Piccoli L. (2019). A single T cell epitope drives the neutralizing anti-drug antibody response to natalizumab in multiple sclerosis patients. Nature medicine, 25(9), 1402-1407.
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