Mkn 45

Cell lines HGC-27 and AGS were obtained from the Cell Bank, Type Culture Collection, Chinese Academy of Sciences, Cell lines HS-746T and MKN-45 were obtained from Shanghai Zhong Qiao Xin Zhou Biotechnology, authenticated via STR profiling. HGC-27 cells were cultured in RPMI-1640 medium with 10% fetal bovine serum, while AGS cells were cultured in DMEM/F12 medium with 10% fetal bovine serum, HS-746T and MKN-45 were cultured in a dedicated medium. These cell lines were maintained in a 5% CO2 incubator at 37°C. Cells were passaged upon reaching 80%-90% confluence using 0.25% trypsin.

Human normal gastric mucosa epithelial cell line GES-1 and poorly differentiation gastric cancer cell line MKN-45 were purchased from Shanghai Zhong Qiao Xin Zhou Biotechnology company. Human undifferentiated gastric cancer cell line HGC-27 was donated by Harbin Medical University and well differentiation gastric cancer cell line
AGS was donated from East China Normal University. Notably, all cells were cultured in RPMI-1640 culture medium (Biological Industries; Kibbutz Beit-Haemek, Israel) containing 10% fetal bovine serum (Biological Industries; Kibbutz Beit-Haemek, Israel) and 1% penicillin-streptomycin (Solarbio; Beijing, China) at 37°C in a 5% CO2 incubator.